Any feedback?
Literature summary for 2.3.1.196 extracted from
- Functional characterization of two acyltransferases from Populus trichocarpa capable of synthesizing benzyl benzoate and salicyl benzoate, potential intermediates in salicinoid phenolic glycoside biosynthesis (2015), Phytochemistry, 113, 149-159.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Populus trichocarpa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H166A | HTMSD motif altered to ATMSD. 97% loss of activity | Populus trichocarpa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.044 | - |
salicyl alcohol | cosubstrate benzoyl-CoA, pH 7.0, 30°C | Populus trichocarpa |  |
| 0.046 | - |
benzoyl-CoA | cosubstrate salicyl alcohol, pH 7.0, 30°C | Populus trichocarpa | |
| 0.052 | - |
benzoyl-CoA | cosubstrate benzyl alcohol, pH 7.0, 30°C | Populus trichocarpa | |
| 0.057 | - |
3-Hydroxybenzyl alcohol | cosubstrate benzoyl-CoA, pH 7.0, 30°C | Populus trichocarpa | |
| 0.059 | - |
benzyl alcohol | cosubstrate benzoyl-CoA, pH 7.0, 30°C | Populus trichocarpa | |
| 0.063 | - |
benzoyl-CoA | cosubstrate 3-hydroxybenzyl alcohol, pH 7.0, 30°C | Populus trichocarpa | |
| 0.067 | - |
3-Hydroxybenzyl alcohol | cosubstrate benzoyl-CoA, pH 7.0, 30°C | Populus trichocarpa | |
| 0.246 | - |
acetyl-CoA | cosubstrate salicyl alcohol, pH 7.0, 30°C | Populus trichocarpa | |
| 0.339 | - |
salicyl alcohol | cosubstrate acetyl-CoA, pH 7.0, 30°C | Populus trichocarpa | |
| 0.365 | - |
acetyl-CoA | cosubstrate benzyl alcohol, pH 7.0, 30°C | Populus trichocarpa | |
| 0.395 | - |
benzyl alcohol | cosubstrate acetyl-CoA, pH 7.0, 30°C | Populus trichocarpa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51900 | - |
x * 51900, calculated and SDS-PAGE | Populus trichocarpa |
| 52600 | - |
x * 52600, calculated and SDS-PAGE | Populus trichocarpa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Populus trichocarpa | A0A0B4VSM7 | - |
- |
| Populus trichocarpa | B9I6S9 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Populus trichocarpa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + 3-hydroxybenzyl alcohol | 41% of the activity with benzoyl-CoA | Populus trichocarpa | 3-hydroxybenzyl acetate + CoA | - |
? | |
| acetyl-CoA + benzyl alcohol | 13% of the activity with benzoyl-CoA | Populus trichocarpa | benzyl acetate + CoA | - |
? | |
| acetyl-CoA + benzyl alcohol | 51% of the activity with benzoyl-CoA | Populus trichocarpa | benzyl acetate + CoA | - |
? | |
| acetyl-CoA + salicyl alcohol | 43.5% of the activity with benzoyl-CoA | Populus trichocarpa | salicyl acetate + CoA | - |
? | |
| benzoyl-CoA + 3-hydroxybenzyl alcohol | 112% of the activity with benzyl alcohol | Populus trichocarpa | 3-hydroxybenzyl benzoate + CoA | - |
? | |
| benzoyl-CoA + 3-hydroxybenzyl alcohol | 97% of the activity with salicyl alcohol | Populus trichocarpa | 3-hydroxybenzyl benzoate + CoA | - |
? | |
| benzoyl-CoA + benzyl alcohol | - |
Populus trichocarpa | benzyl benzoate + CoA | - |
? | |
| benzoyl-CoA + cinnamyl alcohol | 44% of the activity with salicyl alcohol | Populus trichocarpa | cinnamyl benzoate + CoA | - |
? | |
| benzoyl-CoA + coniferyl alcohol | 71% of the activity with salicyl alcohol | Populus trichocarpa | coniferyl benzoate + CoA | - |
? | |
| benzoyl-CoA + salicyl alcohol | - |
Populus trichocarpa | salicyl benzoate + CoA | - |
? | |
| cinnamoyl-CoA + 3-benzyl alcohol | 28.6% of the activity with benzoyl-CoA | Populus trichocarpa | benzyl cinnamate + CoA | - |
? | |
| cinnamoyl-CoA + salicyl alcohol | 11.5% of the activity with benzoyl-CoA | Populus trichocarpa | salicyl cinnamoate + CoA | - |
? | |
| additional information | no substrates: cinnamoyl-CoA, coumaroyl-CoA, or caffeoyl-CoA | Populus trichocarpa | ? | - |
? | |
| additional information | no substrates: coumaroyl-CoA, or caffeoyl-CoA | Populus trichocarpa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 51900, calculated and SDS-PAGE | Populus trichocarpa |
| ? | x * 52600, calculated and SDS-PAGE | Populus trichocarpa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACT47 | - |
Populus trichocarpa |
| ACT49 | - |
Populus trichocarpa |
| BEBT | - |
Populus trichocarpa |
| benzoyl-CoA: salicyl alcohol O-benzoyltransferase | - |
Populus trichocarpa |
| SABT | - |
Populus trichocarpa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.32 | - |
acetyl-CoA | cosubstrate benzyl alcohol, pH 7.0, 30°C | Populus trichocarpa | |
| 0.35 | - |
salicyl alcohol | cosubstrate acetyl-CoA, pH 7.0, 30°C | Populus trichocarpa | |
| 0.4 | - |
benzyl alcohol | cosubstrate acetyl-CoA, pH 7.0, 30°C | Populus trichocarpa | |
| 0.43 | - |
acetyl-CoA | cosubstrate salicyl alcohol, pH 7.0, 30°C | Populus trichocarpa | |
| 1.42 | - |
3-Hydroxybenzyl alcohol | cosubstrate benzoyl-CoA, pH 7.0, 30°C | Populus trichocarpa | |
| 1.72 | - |
benzoyl-CoA | cosubstrate benzyl alcohol, pH 7.0, 30°C | Populus trichocarpa | |
| 1.79 | - |
benzyl alcohol | cosubstrate benzoyl-CoA, pH 7.0, 30°C | Populus trichocarpa | |
| 1.97 | - |
3-Hydroxybenzyl alcohol | cosubstrate benzoyl-CoA, pH 7.0, 30°C | Populus trichocarpa | |
| 2.01 | - |
benzoyl-CoA | cosubstrate 3-hydroxybenzyl alcohol, pH 7.0, 30°C | Populus trichocarpa | |
| 2.06 | - |
salicyl alcohol | cosubstrate benzoyl-CoA, pH 7.0, 30°C | Populus trichocarpa | |
| 2.06 | - |
benzoyl-CoA | cosubstrate salicyl alcohol, pH 7.0, 30°C | Populus trichocarpa | |
| 2.25 | - |
benzoyl-CoA | cosubstrate 3-hydroxybenzyl alcohol, pH 7.0, 30°C | Populus trichocarpa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | - |
Populus trichocarpa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | at least 50% of maximum activity | Populus trichocarpa |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
acetyl-CoA | cosubstrate benzyl alcohol, pH 7.0, 30°C | Populus trichocarpa | |
| 1 | - |
benzyl alcohol | cosubstrate acetyl-CoA, pH 7.0, 30°C | Populus trichocarpa | |
| 1 | - |
salicyl alcohol | cosubstrate acetyl-CoA, pH 7.0, 30°C | Populus trichocarpa | |
| 1.7 | - |
acetyl-CoA | cosubstrate salicyl alcohol, pH 7.0, 30°C | Populus trichocarpa | |
| 30.4 | - |
benzyl alcohol | cosubstrate benzoyl-CoA, pH 7.0, 30°C | Populus trichocarpa | |
| 30.4 | - |
3-Hydroxybenzyl alcohol | cosubstrate benzoyl-CoA, pH 7.0, 30°C | Populus trichocarpa | |
| 32.1 | - |
benzoyl-CoA | cosubstrate 3-hydroxybenzyl alcohol, pH 7.0, 30°C | Populus trichocarpa | |
| 32.6 | - |
3-Hydroxybenzyl alcohol | cosubstrate benzoyl-CoA, pH 7.0, 30°C | Populus trichocarpa | |
| 33.2 | - |
benzoyl-CoA | cosubstrate benzyl alcohol, pH 7.0, 30°C | Populus trichocarpa | |
| 35.5 | - |
benzoyl-CoA | cosubstrate 3-hydroxybenzyl alcohol, pH 7.0, 30°C | Populus trichocarpa | |
| 45 | - |
benzoyl-CoA | cosubstrate salicyl alcohol, pH 7.0, 30°C | Populus trichocarpa | |
| 46.6 | - |
salicyl alcohol | cosubstrate benzoyl-CoA, pH 7.0, 30°C | Populus trichocarpa | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
