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Literature summary for 2.3.1.196 extracted from
- Characterization of an acyltransferase capable of synthesizing benzyl benzoate and other volatile esters in flowers and damaged leaves of Clarkia breweri (2002), Plant Physiol., 130, 466-476.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Clarkia breweri |
| expression in Escherichia coli | Nicotiana tabacum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM, strong inhibition | Clarkia breweri |  |
| Co2+ | 5 mM, strong inhibition | Clarkia breweri | |
| Cu2+ | 5 mM, strong inhibition | Clarkia breweri | |
| Mg2+ | 5 mM, strong inhibition | Clarkia breweri | |
| Zn2+ | 5 mM, strong inhibition | Clarkia breweri | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
benzyl alcohol | pH 7.7, 22°C, cosustrate: benzoyl-CoA | Nicotiana tabacum | |
| 0.0205 | - |
benzoyl-CoA | pH 7.7, 22°C, cosustrate: benzyl alcohol | Clarkia breweri | |
| 0.035 | - |
benzoyl-CoA | pH 7.7, 22°C, cosustrate: benzyl alcohol | Nicotiana tabacum | |
| 0.0468 | - |
benzyl alcohol | pH 7.7, 22°C, cosustrate: benzoyl-CoA | Clarkia breweri | |
| 0.0978 | - |
cinnamyl alcohol | pH 7.7, 22°C, cosustrate: benzoyl-CoA | Clarkia breweri | |
| 0.464 | - |
cinnamoyl-CoA | pH 7.7, 22°C, cosustrate: benzyl alcohol | Clarkia breweri | |
| 0.818 | - |
acetyl-CoA | pH 7.7, 22°C, cosustrate: benzyl alcohol | Clarkia breweri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | 49500 | gel filtration | Clarkia breweri |
| 50650 | - |
calculated from sequence | Clarkia breweri |
| 51010 | - |
calculated from sequence | Nicotiana tabacum |
| 51014 | - |
x * 51014, calculated from sequence | Nicotiana tabacum |
| 55000 | - |
1 * 55000, SDS-PAGE | Clarkia breweri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-CoA + benzyl alcohol | Clarkia breweri | the enzyme catalyzes the formation of benzyl benzoate, a minor constituent of the Clarkia breweri floral aroma | benzyl benzoate + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clarkia breweri | Q8GT21 | - |
- |
| Nicotiana tabacum | Q8GT20 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| complete, non-fusion BEBT enzyme from the crude Escherichia coli extract | Clarkia breweri |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | the BEBT gene is expressed in different parts of the flowers with maximal RNA transcript levels in the stigma | Clarkia breweri | - |
| leaf | induced upon wounding | Nicotiana tabacum | - |
| leaf | no expression in the leaves under normal conditions. BEBT expression is induced in damaged leaves, reaching a maximum 6 h after damage occurres | Clarkia breweri | - |
| petal | 30% of the activity in stigma | Clarkia breweri | - |
| sepal | 30% of the activity in stigma | Clarkia breweri | - |
| stamen | 30% of the activity in stigma | Clarkia breweri | - |
| stigma | the BEBT gene is expressed in different parts of the flowers with maximal RNA transcript levels in the stigma | Clarkia breweri | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + benzyl alcohol | the Km-value for acetyl-CoA (0.818 mM) strongly suggests that acetyl-CoA is not commonly used by BEBT as the acyl donor in vivo | Clarkia breweri | benzyl acetate + CoA | - |
? | |
| benzoyl-CoA + benzyl alcohol | - |
Clarkia breweri | benzyl benzoate + CoA | - |
? | |
| benzoyl-CoA + benzyl alcohol | - |
Nicotiana tabacum | benzyl benzoate + CoA | - |
? | |
| benzoyl-CoA + benzyl alcohol | the enzyme catalyzes the formation of benzyl benzoate, a minor constituent of the Clarkia breweri floral aroma | Clarkia breweri | benzyl benzoate + CoA | - |
? | |
| benzoyl-CoA + cinnamyl alcohol | - |
Clarkia breweri | cinnamyl benzoate + CoA | - |
? | |
| cinnamoyl-CoA + benzyl alcohol | the Km-value for cinnamoyl-CoA (0.464 mM) strongly suggests that acetyl-CoA is not commonly used by BEBT as the acyl donor in vivo | Clarkia breweri | ? + CoA | - |
? | |
| additional information | coumaroyl-CoA does not act as substrate | Clarkia breweri | ? | - |
? | |
| additional information | no activity with acetyl-CoA at concentrations below 1 mM | Nicotiana tabacum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 51014, calculated from sequence | Nicotiana tabacum |
| monomer | 1 * 55000, SDS-PAGE | Clarkia breweri |
| monomer | 1 * 50650, calculated from sequence | Clarkia breweri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BEBT | - |
Clarkia breweri |
| benzoyl-CoA:benzyl alcohol benzoyl transferase | - |
Clarkia breweri |
| benzoyl-coenzyme A:benzyl alcohol benzoyl transferase | - |
Clarkia breweri |
| HSR201 | - |
Nicotiana tabacum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Clarkia breweri |
| 22 | - |
assay at | Nicotiana tabacum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
30 min, stable | Clarkia breweri |
| 37 | - |
30 min, 20% loss of activity | Clarkia breweri |
| 50 | - |
30 min, complete inactivation | Clarkia breweri |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
cinnamoyl-CoA | pH 7.7, 22°C, cosustrate: benzyl alcohol | Clarkia breweri | |
| 21.5 | - |
acetyl-CoA | pH 7.7, 22°C, cosustrate: benzyl alcohol | Clarkia breweri | |
| 47.2 | - |
cinnamyl alcohol | pH 7.7, 22°C, cosustrate: benzoyl-CoA | Clarkia breweri | |
| 85.2 | - |
benzyl alcohol | pH 7.7, 22°C, cosustrate: benzoyl-CoA | Clarkia breweri | |
| 124 | - |
benzoyl-CoA | pH 7.7, 22°C, cosustrate: benzyl alcohol | Clarkia breweri | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
- |
Clarkia breweri |
| 7.7 | - |
assay at | Nicotiana tabacum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | pH 6.5: 39% of maximal activity, pH 9.0: 69% of maximal activity | Clarkia breweri |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme catalyzes the formation of benzyl benzoate, a minor constituent of the Clarkia breweri floral aroma | Clarkia breweri |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
cinnamoyl-CoA | pH 7.7, 22°C, cosustrate: benzyl alcohol | Clarkia breweri | |
| 2.5 | - |
benzoyl-CoA | pH 7.7, 22°C, cosustrate: benzyl alcohol | Clarkia breweri | |
| 4 | - |
benzyl alcohol | pH 7.7, 22°C, cosustrate: benzoyl-CoA | Clarkia breweri | |
| 4.6 | - |
cinnamyl alcohol | pH 7.7, 22°C, cosustrate: benzoyl-CoA | Clarkia breweri | |
| 17.5 | - |
acetyl-CoA | pH 7.7, 22°C, cosustrate: benzyl alcohol | Clarkia breweri | |
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