Cloned (Comment) | Organism |
---|---|
component E1alpha | Saccharolobus solfataricus |
component E1beta | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
acetoin | E1 complex, 80°C, pH 7.0 | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
142000 | - |
gel filtration, E1 complex | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97YF5 | E1beta subunit | - |
Saccharolobus solfataricus | Q97YF6 | E1alpha subunit | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoin + 2,6-dichlorophenolindophenol | E1 complex | Saccharolobus solfataricus | acetaldehyde + ? | - |
? | |
diacetyl + 2,6-dichlorophenolindophenol | E1 complex, 59% of the activity with acetoin | Saccharolobus solfataricus | ? | - |
? | |
methyl acetoin + 2,6-dichlorophenolindophenol | E1 complex, 107% of the activity with acetoin | Saccharolobus solfataricus | ? + ? | - |
? | |
additional information | no detectable activity with 2-oxoglutarate, pyruvate or the branched-chain 2-oxoacids, 4-methyl-2-oxopentanoate, 3-methyl-2-oxopentanoate and 3-methyl-2-oxobutanoate | Saccharolobus solfataricus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | component E1, i.e. acetoin:2,6-dichlorophenolindophenol oxidoreductase, exhibits tetrameric alpha2beta2 structure, with alpha, 43000 and beta, 33000 Da, SDS-PAGE | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 85 | - |
Saccharolobus solfataricus |