Cloned (Comment) | Organism |
---|---|
the Rv0819 gene encoding MshD (residues 1315) is amplified from Mycobacterium tuberculosis genomic DNA using PCR and cloned into the pET28a(+) plasmid. This vector construction adds six histidine residues and a thrombin cleavage sequence to the N-terminus to facilitate protein purification. Expression in Escherichia coli stain BL21(DE3)pLysS | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
vapor diffusion under oil method | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
desacetylmycothiol + acetyl-CoA | Mycobacterium tuberculosis | - |
mycothiol + coenzyme-A | - |
? | |
desacetylmycothiol + acetyl-CoA | Mycobacterium tuberculosis H37Rv | - |
mycothiol + coenzyme-A | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WJM7 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WJM7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
desacetylmycothiol + acetyl-CoA | - |
Mycobacterium tuberculosis | mycothiol + coenzyme-A | - |
? | |
desacetylmycothiol + acetyl-CoA | - |
Mycobacterium tuberculosis H37Rv | mycothiol + coenzyme-A | - |
? |