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Literature summary for 2.3.1.188 extracted from

  • Lotfy, S.; Javelle, F.; Negrel, J.
    Purification and characterization of hydroxycinnamoyl-coenzyme A:omega-hydroxypalmitic acid O-hydroxycinnamoyltransferase from tobacco (Nicotiana tabacum) cell-suspension cultures (1996), Planta, 199, 475-480.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
16-hydroxypalmitic acid pH 7.0, 30°C Nicotiana tabacum
0.0007
-
hexadecan-1,16-diol pH 7.0, 30° Nicotiana tabacum
0.001
-
1-Tetradecanol pH 7.0, 30°C Nicotiana tabacum
0.002
-
1-hexadecanol pH 7.0, 30°C Nicotiana tabacum
0.002
-
1-Dodecanol pH 7.0, 30°C Nicotiana tabacum
0.0033
-
sinapoyl-CoA pH 7.0, 30°C Nicotiana tabacum
0.01
-
p-Coumaroyl-CoA pH 7.0, 30°C Nicotiana tabacum
0.0112
-
15-hydroxypentadecanoic acid pH 7.0, 30°C Nicotiana tabacum
0.015
-
feruloyl-CoA pH 7.0, 30°C Nicotiana tabacum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
x * 55000, SDS-PAGE Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Nicotiana tabacum

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture late logarithmic phase Nicotiana tabacum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
feruloyl-CoA + 1-dodecanol 49% of the activity with 16-hydroxypalmitic acid Nicotiana tabacum CoA + (E)-dodecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
?
feruloyl-CoA + 1-hexadecanol
-
Nicotiana tabacum CoA + (E)-hexadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
?
feruloyl-CoA + 1-pentadecanol 53% of the activity with 16-hydroxypalmitic acid Nicotiana tabacum CoA + (E)-pentadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
?
feruloyl-CoA + 1-tetradecanol 82% of the activity with 16-hydroxypalmitic acid Nicotiana tabacum CoA + (E)-tetradecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
?
feruloyl-CoA + 15-hydroxypentadecanoic acid 68% of the activity with 16-hydroxypalmitate Nicotiana tabacum CoA + (E)-15-(3-(4-hydroxy-3-methoxyphenyl)acryloyloxy)pentadecanoic acid
-
?
feruloyl-CoA + 16-hydroxypalmitic acid
-
Nicotiana tabacum CoA + (E)-16-(3-(4-hydroxy-3-methoxyphenyl)acryloyloxy)hexadecanoic acid
-
?
feruloyl-CoA + hexadecane-1,16-diol 52% of the activity with 16-hydroxypalmitic acid Nicotiana tabacum CoA + (E)-16-hydroxyhexadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
?
additional information no substrate: 18-hydroxy-cis-9-octadecenoic acid Nicotiana tabacum ?
-
?
p-coumaroyl-CoA + 16-hydroxypalmitic acid 51% of the activity with feruloyl-CoA Nicotiana tabacum CoA + (E)-16-(3-(4-hydroxyphenyl)acryloyloxy)hexadecanoic acid
-
?
sinapoyl-CoA + 16-hydroxypalmitic acid 50% of the activity with feruloyl-CoA Nicotiana tabacum CoA + (E)-16-(3-(4-hydroxy-3,5-dimethoxyphenyl)acryloyloxy)hexadecanoic acid
-
?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Nicotiana tabacum

pI Value

Organism Comment pI Value Maximum pI Value
Nicotiana tabacum isoelectric focusing
-
4.6