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Literature summary for 2.3.1.188 extracted from

  • Lotfy, S.; Negrel, J.; Javelle, F.
    Formation of omega-feruloyloxypalmitic acid by an enzyme from wound-healing potato tuber disks (1994), Phytochemistry, 35, 1419-1424.
No PubMed abstract available
Search for more literature for 2.3.1.188

Inhibitors

Inhibitors Comment Organism Structure
Tris-HCl 60% of maximal activity in Tris-HCl compared with potassium phosphate buffer Solanum tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 16-hydroxypalmitic acid pH 7.0, 30°C Solanum tuberosum
0.0017
-
 hexadecan-1,16-diol pH 7.0, 30°C Solanum tuberosum
0.004
-
 1-Tetradecanol pH 7.0, 30°C Solanum tuberosum
0.005
-
 feruloyl-CoA pH 7.0, 30°C Solanum tuberosum
0.005
-
 1-Dodecanol pH 7.0, 30°C Solanum tuberosum
0.0071
-
 sinapoyl-CoA pH 7.0, 30°C Solanum tuberosum
0.0095
-
 1-pentadecanol pH 7.0, 30°C Solanum tuberosum
0.01
-
 1-hexadecanol pH 7.0, 30°C Solanum tuberosum
0.0143
-
 p-Coumaroyl-CoA pH 7.0, 30°C Solanum tuberosum
0.0168
-
 15-hydroxypentadecanoic acid pH 7.0, 30°C Solanum tuberosum

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Solanum tuberosum

Source Tissue

Source Tissue Comment Organism Textmining
tuber wound-healing tuber disc Solanum tuberosum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
feruloyl-CoA + 1-dodecanol 49% of the activity with 16-hydroxypalmitic acid Solanum tuberosum CoA + (E)-dodecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
 ?
feruloyl-CoA + 1-hexadecanol 52% of the activity with 16-hydroxypalmitic acid Solanum tuberosum CoA + (E)-hexadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
 ?
feruloyl-CoA + 1-pentadecanol 53% of the activity with 16-hydroxypalmitic acid Solanum tuberosum CoA + (E)-pentadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
 ?
feruloyl-CoA + 1-tetradecanol 82% of the activity with 16-hydroxypalmitic acid Solanum tuberosum CoA + (E)-tetradecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
-
 ?
feruloyl-CoA + 15-hydroxypentadecanoic acid 68% of the activity with 16-hydroxypalmitate Solanum tuberosum CoA + (E)-15-(3-(4-hydroxy-3-methoxyphenyl)acryloyloxy)pentadecanoic acid
-
 ?
feruloyl-CoA + 16-hydroxypalmitic acid
-
 Solanum tuberosum CoA + (E)-16-(3-(4-hydroxy-3-methoxyphenyl)acryloyloxy)hexadecanoic acid
-
 ?
feruloyl-CoA + hexadecane-1,16-diol 52% of the activity with 16-hydroxypalmitic acid Solanum tuberosum CoA + (E)-16-hydroxyhexadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate no formation of the diferulate ?
p-coumaroyl-CoA + 16-hydroxypalmitic acid 51% of the activity with feruloyl-CoA Solanum tuberosum CoA + (E)-16-(3-(4-hydroxyphenyl)acryloyloxy)hexadecanoic acid
-
 ?
sinapoyl-CoA + 16-hydroxypalmitic acid 50% of the activity with feruloyl-CoA Solanum tuberosum CoA + (E)-16-(3-(4-hydroxy-3,5-dimethoxyphenyl)acryloyloxy)hexadecanoic acid
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 potassium phosphate buffer, 60% of maximal activity in Tris-HCl Solanum tuberosum

pH Range

pH Minimum pH Maximum Comment Organism
5.5
-
 half-maximal activity Solanum tuberosum
7.5
-
 half-maximal activity Solanum tuberosum
10
-
 5% of maximal acitvity Solanum tuberosum