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Literature summary for 2.3.1.186 extracted from

  • Rabot, S.; Peerless, A.C.J.; Robins, R.J.
    Tigloyl-CoA:pseudotropine acyl transferase - an enzyme of tropane alkaloid biosynthesis (1995), Phytochemistry, 39, 315-322.
No PubMed abstract available
Search for more literature for 2.3.1.186

Inhibitors

Inhibitors Comment Organism Structure
acetyl-CoA competitively inhibits activity with tigloyl-CoA Datura stramonium
tigloyl-CoA competitively inhibits activity with acetyl-CoA Datura stramonium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
 acetyl-CoA 30°C, pH 9 Datura stramonium
0.36
-
 pseudotropine 30°C, pH 9 Datura stramonium
1.31
-
 tigloyl-CoA 30°C, pH 9 Datura stramonium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 gel filtration Datura stramonium
66000
-
 1 * 66000, SDS-PAGE Datura stramonium

Organism

Organism UniProt Comment Textmining
Datura stramonium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Datura stramonium

Source Tissue

Source Tissue Comment Organism Textmining
root root culture, activity is higher during rapid growth than in stationary phase roots Datura stramonium
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.6
-
-
 Datura stramonium

Storage Stability

Storage Stability Organism
-20°C, stable for at least 18 months Datura stramonium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylcrotonyl-CoA + pseudotropine 4% of the activity with tigloyl-CoA Datura stramonium CoA + O-(3-methylcrotonyl)pseudotropine
-
 ?
acetyl-CoA + pseudotropine 4% of the activity with tigloyl-CoA Datura stramonium CoA + O-acetylpseudotropine
-
 ?
isobutyryl-CoA + pseudotropine 10% of the activity with tigloyl-CoA Datura stramonium CoA + O-isobutyrylpseudotropine
-
 ?
isovaleryl-CoA + pseudotropine 2% of the activity with tigloyl-CoA Datura stramonium CoA + O-isovalerylpseudotropine
-
 ?
additional information tropine and norpseudotropine are not acylated Datura stramonium ?
-
 ?
n-propionyl-CoA + pseudotropine 5% of the activity with tigloyl-CoA Datura stramonium CoA + O-n-propionylpseudotropine
-
 ?
tigloyl-CoA + 4-hydroxy-1-methylpiperidine 14% of the activity with pseudotropine Datura stramonium CoA + O-tigloyl-4-hydroxy-1-methylpiperidine
-
 ?
tigloyl-CoA + pseudotropine
-
 Datura stramonium CoA + O-tigloylpseudotropine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 66000, SDS-PAGE Datura stramonium

Synonyms

Synonyms Comment Organism
acetyl-CoA:pseudotropine acyl transferase
-
 Datura stramonium
tigloyl-CoA:pseudotropine acyl transferase
-
 Datura stramonium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 activity is highest in glycine buffer, lowerv activity in Tris and borate buffer Datura stramonium

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 pH 7.0: about 35% of maximal activity, pH 10.0: about 20% of 20% of maximal activity Datura stramonium

pI Value

Organism Comment pI Value Maximum pI Value
Datura stramonium chromatofocusing
-
 5.4