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Literature summary for 2.3.1.184 extracted from

  • Gould, T.A.; Schweizer, H.P.; Churchill, M.E.
    Structure of the Pseudomonas aeruginosa acyl-homoserinelactone synthase LasI (2004), Mol. Microbiol., 53, 1135-1146.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
functional overexpression of His-tagged LasIDELTAG and point mutants in Escherichia coli as soluble proteins Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant detagged LasIDELTAG, hanging drop vapour diffusion method, from 1.5 M ammonium sulfate, 0.125 M disodium sulfate, and 0.1 M MOPS, pH 6.5, cryoprotection with 15% glycerol, heavy atom derivatization with Hg2+, single isomorphous replacement with anomalous scattering, X-ray diffraction structure determination and analysis at 2.3-3.1 A resolution, structure modeling Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
K150E/R154QE site-directed mutagenesis, the mutant shows highly reduced activity compared to the fully active mutant LasIDELTAG Pseudomonas aeruginosa
K150Q site-directed mutagenesis, the mutant shows similar activity as the fully active mutant LasIDELTAG Pseudomonas aeruginosa
K150Q/R154Q site-directed mutagenesis, the mutant shows reduced activity compared to the fully active mutant LasIDELTAG Pseudomonas aeruginosa
R154E site-directed mutagenesis, the mutant shows reduced activity compared to the fully active mutant LasIDELTAG Pseudomonas aeruginosa
R154Q site-directed mutagenesis, the mutant shows similar activity as the fully active mutant LasIDELTAG Pseudomonas aeruginosa
R172A site-directed mutagenesis, the mutant shows increased activity compared to the fully active mutant LasIDELTAG Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas aeruginosa LasI and LasR form a quorum-sensing system playing a pivotal role in virulence gene regulation of the opportunistic human pathogen Pseudomonas aeruginosa ?
-
?
S-adenosyl-L-methionine + 3-oxododecanoyl-[acyl-carrier protein] Pseudomonas aeruginosa substrate of LasI 5'-methylthioadenosine + N-3-oxododecanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
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?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
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gene lasI
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Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged LasIDELTAG from Escherichia coli by nickel affinity chromatography and gel filtration, the His-tag is removed by thrombin cleavage Pseudomonas aeruginosa

Reaction

Reaction Comment Organism Reaction ID
an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone substrate binding structure Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activities of mutant LasI enzymes Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information LasI and LasR form a quorum-sensing system playing a pivotal role in virulence gene regulation of the opportunistic human pathogen Pseudomonas aeruginosa Pseudomonas aeruginosa ?
-
?
S-adenosyl-L-methionine + 3-oxododecanoyl-[acyl-carrier protein] substrate of LasI Pseudomonas aeruginosa 5'-methylthioadenosine + N-3-oxododecanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
?

Synonyms

Synonyms Comment Organism
acyl-homoserinelactone synthase
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Pseudomonas aeruginosa
AHL synthase
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Pseudomonas aeruginosa
LasI
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Pseudomonas aeruginosa
LasR
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Pseudomonas aeruginosa