Literature summary for 2.3.1.184 extracted from

Brader, G.; Sjoblom, S.; Hyytiainen, H.; Sims-Huopaniemi, K.; Palva, E.T.
Altering substrate chain length specificity of an acylhomoserine lactone synthase in bacterial communication (2005), J. Biol. Chem., 280, 10403-10409.

Search for more literature for 2.3.1.184

Cloned(Commentary)

Cloned (Comment)	Organism
gene expISCC1, expression of wild-type and mutant enzymes in Escherichai coli, inoculation of Solanum tuberosum tubers	Pectobacterium carotovorum subsp. carotovorum

Protein Variants

Protein Variants	Comment	Organism
F69L	site-directed mutagenesis of ExpISCC1, the mutant shows altered substrate acyl-chain lemgth specificity compared to the wild-type enzyme	Pectobacterium carotovorum subsp. carotovorum
M127T	site-directed mutagenesis of ExpISCC1, the mutant shows altered substrate acyl-chain lemgth specificity compared to the wild-type enzyme	Pectobacterium carotovorum subsp. carotovorum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + 3-oxohexanoyl-[acyl-carrier protein]	Pectobacterium carotovorum subsp. carotovorum	substrate of wild-type ExpISCC1, specific for	5'-methylthioadenosine + N-3-oxohexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]	-	?
S-adenosyl-L-methionine + 3-oxooctanoyl-[acyl-carrier protein]	Pectobacterium carotovorum subsp. carotovorum	substrate of wild-type ExpISCC3065, specific for	5'-methylthioadenosine + N-3-oxooctanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]	-	?

Organism

Organism	UniProt	Comment	Textmining
Pectobacterium carotovorum subsp. carotovorum	Q6R8K9	i.e. Pectobacterium carotovora ssp. carotovora, genes expISCC1 and expISCC3065	-

Reaction

Reaction	Comment	Organism	Reaction ID
an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone	molecular basis of acyl chain length specificity	Pectobacterium carotovorum subsp. carotovorum	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	substrate specificities of wild-type and mutant ExpISCC1 and ExpISCC3065 enzymes	Pectobacterium carotovorum subsp. carotovorum	?	-	?
S-adenosyl-L-methionine + 3-oxohexanoyl-[acyl-carrier protein]	substrate of wild-type ExpISCC1, specific for	Pectobacterium carotovorum subsp. carotovorum	5'-methylthioadenosine + N-3-oxohexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]	-	?
S-adenosyl-L-methionine + 3-oxooctanoyl-[acyl-carrier protein]	substrate of wild-type ExpISCC3065, specific for	Pectobacterium carotovorum subsp. carotovorum	5'-methylthioadenosine + N-3-oxooctanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]	-	?
S-adenosyl-L-methionine + hexanoyl-[acyl-carrier protein]	substrate of wild-type ExpISCC1, very low activity	Pectobacterium carotovorum subsp. carotovorum	5'-methylthioadenosine + N-hexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]	-	?
S-adenosyl-L-methionine + octanoyl-[acyl-carrier protein]	substrate of wild-type ExpISCC3065, very low activity	Pectobacterium carotovorum subsp. carotovorum	5'-methylthioadenosine + N-octanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]	-	?

Synonyms

Synonyms	Comment	Organism
acylhomoserine lactone synthase	-	Pectobacterium carotovorum subsp. carotovorum
AHSL synthase	-	Pectobacterium carotovorum subsp. carotovorum
ExpISCC1	-	Pectobacterium carotovorum subsp. carotovorum
ExpISCC3065	-	Pectobacterium carotovorum subsp. carotovorum

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Release 2023.1 (January 2023)