Cloned (Comment) | Organism |
---|---|
gene ainS, expression of AinS as maltose binding protein-fusion enzyme in Escherichia coli strain XL 1-Blue | Aliivibrio fischeri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-methylthioadenosine | product inhibition, 51% inhibition at 0.005 mM, 92% inhibition at 0.06 mM | Aliivibrio fischeri | |
S-Adenosyl-D-homocysteine | 50% inhibition at 0.25 mM, 85% inhibition at 2.5 mM | Aliivibrio fischeri | |
S-adenosyl-L-homocysteine | 16% inhibition at 0.25 mM, 45% inhibition at 2.5 mM | Aliivibrio fischeri | |
[Acyl-carrier-protein] | product inhibition, in presence of 10 mM DTT, 26% inhibition at 0.25 mM, 43% inhibition at 0.5 mM | Aliivibrio fischeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Aliivibrio fischeri | |
0.004 | - |
octanoyl-[acyl-carrier protein] | pH 8.5, 25°C, recombinant MBP-AinS | Aliivibrio fischeri | |
0.015 | - |
octanoyl-CoA | pH 8.5, 25°C, recombinant MBP-AinS | Aliivibrio fischeri | |
0.023 | - |
S-adenosyl-L-methionine | pH 8.5, 25°C, recombinant MBP-AinS, with octanoyl-[acyl-carrier-protein] | Aliivibrio fischeri | |
0.061 | - |
S-adenosyl-L-methionine | pH 8.5, 25°C, recombinant MBP-AinS, with octanoyl-CoA | Aliivibrio fischeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87000 | - |
x * 87000, recombinant MBP-AinS, SDS-PAGE | Aliivibrio fischeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3-hydroxybutyryl-[acyl-carrier protein] | Aliivibrio fischeri | substrate of LuxM | 5'-methylthioadenosine + N-3-hydroxybutyryl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? | |
S-adenosyl-L-methionine + 3-oxohexanoyl-[acyl-carrier protein] | Aliivibrio fischeri | substrate of LuxI | 5'-methylthioadenosine + N-3-oxohexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? | |
S-adenosyl-L-methionine + octanoyl-[acyl-carrier protein] | Aliivibrio fischeri | substrate of AinS | 5'-methylthioadenosine + N-octanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aliivibrio fischeri | - |
genes ainS, luxM, and luxI | - |
Purification (Comment) | Organism |
---|---|
recobinant maltose binding protein-fusion AinS from Escherichia coli by amylose affinity chromatography | Aliivibrio fischeri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Aliivibrio fischeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity of AinS, weak activity with S-adenosyl-L-ethionine, and butyryl-[acyl-carrier-protein], no activity with sodium octanoate | Aliivibrio fischeri | ? | - |
? | |
S-adenosyl-L-methionine + 3-hydroxybutyryl-[acyl-carrier protein] | substrate of LuxM | Aliivibrio fischeri | 5'-methylthioadenosine + N-3-hydroxybutyryl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? | |
S-adenosyl-L-methionine + 3-oxohexanoyl-[acyl-carrier protein] | substrate of LuxI | Aliivibrio fischeri | 5'-methylthioadenosine + N-3-oxohexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? | |
S-adenosyl-L-methionine + decanoyl-[acyl-carrier protein] | substrate of recombinant MBP-AinS | Aliivibrio fischeri | 5'-methylthioadenosine + N-decanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? | |
S-adenosyl-L-methionine + hexanoyl-[acyl-carrier protein] | substrate of recombinant MBP-AinS | Aliivibrio fischeri | 5'-methylthioadenosine + N-hexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? | |
S-adenosyl-L-methionine + octanoyl-CoA | substrate of recombinant MBP-AinS, lower activity | Aliivibrio fischeri | 5'-methylthioadenosine + N-octanoyl-L-homoserine-1,4-lactone + CoA | - |
? | |
S-adenosyl-L-methionine + octanoyl-[acyl-carrier protein] | substrate of AinS | Aliivibrio fischeri | 5'-methylthioadenosine + N-octanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? | |
S-adenosyl-L-methionine + octanoyl-[acyl-carrier protein] | preferred substrate of native AinS and recombinant MBP-AinS | Aliivibrio fischeri | 5'-methylthioadenosine + N-octanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 87000, recombinant MBP-AinS, SDS-PAGE | Aliivibrio fischeri |
Synonyms | Comment | Organism |
---|---|---|
acylhomoserine lactone synthase | - |
Aliivibrio fischeri |
AinS | - |
Aliivibrio fischeri |
AinS protein | - |
Aliivibrio fischeri |
LuxI | - |
Aliivibrio fischeri |
LuxM | - |
Aliivibrio fischeri |
More | the enzymes are members of the LuxI family of synthases | Aliivibrio fischeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
- |
Aliivibrio fischeri |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 42 | over 60% of maximal activity at 25°C and 37°C, less than 15% of maximal activity at 15°C and 42°C | Aliivibrio fischeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Aliivibrio fischeri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | sharp drop of activity below pH 8.0 and above pH 10.0, inactive at pH 6.0 and pH 11.0 | Aliivibrio fischeri |