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Literature summary for 2.3.1.183 extracted from

  • Davies, A.M.; Tata, R.; Beavil, R.L.; Sutton, B.J.; Brown, P.R.
    l-Methionine sulfoximine, but not phosphinothricin, is a substrate for an acetyltransferase (gene PA4866) from Pseudomonas aeruginosa: structural and functional studies (2007), Biochemistry, 46, 1829-1839.
    View publication on PubMed
Search for more literature for 2.3.1.183

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pET24a for expression in Escherichia coli BL21DE3 cells Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
a high-resolution, 1.55 A, crystal structure of pita in complex with L-methionine sulfoximine is solved Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
Depp4 mutant, in which pita is inactivated through an in-frame deletion Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 acetyl-CoA apparent KM Pseudomonas aeruginosa
1.3
-
 L-methionine sulfoximine apparent KM Pseudomonas aeruginosa
1.3
-
 L-methionine sulfone apparent KM Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HUU7 putative uncharacterized protein
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-methionine sulfone
-
 Pseudomonas aeruginosa ?
-
 ?
acetyl-CoA + L-methionine sulfoximine enzyme has no activity towards phosphinothricin Pseudomonas aeruginosa ?
-
 ?

Synonyms

Synonyms Comment Organism
phosphinothricin acetyltransferase
-
 Pseudomonas aeruginosa
PitA
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 activity assay Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
505
-
 L-methionine sulfoximine apparent Kcat Pseudomonas aeruginosa
610
-
 L-methionine sulfone apparent Kcat Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 activity assay Pseudomonas aeruginosa