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Literature summary for 2.3.1.183 extracted from

  • Wohllehen, W.; Arnold, W.; Broer, L.; Hillemaan, D.; Strauch, E.; Ptihler, A.
    Nucleotide sequence of the phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tü494 and its expression in Nicotiana tabacum (1988), Gene, 70, 25-37.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene pat, DNA and amino acid sequence determination and analysis, translation of pat is initiated by a GTG codon, functional expression in transgenic Nicotiana tabacum plants by Agrobacterium tumefaciens strain LBA4404-mediated leaf-disc transformation replacing the GTG start codon by ATG, expression of wild-type and mutant enzymes in Streptomyces lividans strain TK23 and in Escherichia coli strains JM83 and S17.1, the gene confers resistance to L-phosphinothricin Streptomyces viridochromogenes

Protein Variants

Protein Variants Comment Organism
additional information construction of insertion mutants of pat, phenotypes and genetic regulation Streptomyces viridochromogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20621
-
x * 20621, nucleotide sequence calculation Streptomyces viridochromogenes

Organism

Organism UniProt Comment Textmining
Streptomyces viridochromogenes
-
gene pat
-
Streptomyces viridochromogenes Tue494
-
gene pat
-

Subunits

Subunits Comment Organism
? x * 20621, nucleotide sequence calculation Streptomyces viridochromogenes

Synonyms

Synonyms Comment Organism
phosphinothricin N-acetyltransferase
-
Streptomyces viridochromogenes