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Literature summary for 2.3.1.183 extracted from

  • Botterman, J.; Gossele, V.; Thoen, C.; Lauwereys, M.
    Characterization of phosphinothricin acetyltransferase and C-terminal enzymatically active fusion proteins (1991), Gene, 102, 33-37.
    View publication on PubMed

Application

Application Comment Organism
molecular biology the bar gene represents a selectable and assayable reporter gene especially suitable for 3’-terminal gene fusions Streptomyces hygroscopicus

Cloned(Commentary)

Cloned (Comment) Organism
gene bar, expression in Escherichia coli with gene fragments fused to the 3' end, the fusion gene product shows unaltered kinetic properties compared to the unmodified gene product Streptomyces hygroscopicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetic analysis of the recombinant wild-type and modified enzymes Streptomyces hygroscopicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-phosphinothricin Streptomyces hygroscopicus
-
CoA + L-N-acetylphosphinothricin
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces hygroscopicus
-
gene bar
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-phosphinothricin
-
Streptomyces hygroscopicus CoA + L-N-acetylphosphinothricin
-
?

Synonyms

Synonyms Comment Organism
Pat
-
Streptomyces hygroscopicus
PPT acetyltransferase
-
Streptomyces hygroscopicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 55
-
Streptomyces hygroscopicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 55
-
Streptomyces hygroscopicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Streptomyces hygroscopicus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 no activity at pH 5.0 and pH 10.0 Streptomyces hygroscopicus

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
Streptomyces hygroscopicus