Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.181 extracted from

  • Martin, N.; Christensen, Q.H.; Mansilla, M.C.; Cronan, J.E.; de Mendoza, D.
    A novel two-gene requirement for the octanoyltransfer reaction of Bacillus subtilis lipoic acid biosynthesis (2011), Mol. Microbiol., 80, 335-349.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.181

Cloned(Commentary)

Cloned (Comment) Organism
gene lipM, complements a lipB-defective Escherichia coli mutant strain Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information construction of strain NM57 in which lipM is replaced with a kanamycin-resistance determinant. Gene lipM is placed under control of a xylose-inducible promoter PxylA is introduced into the DELTAlipM strain NM57 giving the lipM amyE::PxylA-lipM strain NM08. LipM can be functionally replaced by expression of Escherichia coli lipB, expression of Escherichia coli lipB allows growth of Bacillus subtilis DELTAlipL or DELATlipM strains in the absence of supplements. In contrast, growth of an Escherichia coli DELTAlipB strain can be complemented with lipM, but not lipL Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an octanoyl-[acyl-carrier protein] + a protein Bacillus subtilis
-
 a protein N6-(octanoyl)lysine + an [acyl-carrier protein]
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
 gene lipM
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
an octanoyl-[acyl-carrier protein] + a protein
-
 Bacillus subtilis a protein N6-(octanoyl)lysine + an [acyl-carrier protein]
-
 ?
an octanoyl-[acyl-carrier protein] + a protein acyl-enzyme intermediate mechanism Bacillus subtilis a protein N6-(octanoyl)lysine + an [acyl-carrier protein]
-
 ?

Synonyms

Synonyms Comment Organism
ACP:protein-N-octanoyltransferase
-
 Bacillus subtilis
LipM
-
 Bacillus subtilis
octanoyl-acyl carrier protein:protein-N-octanoyltransferase
-
 Bacillus subtilis

General Information

General Information Comment Organism
malfunction DELTAlipL strains are unable to synthesize lipoic acid despite the presence of LipM and the sulfur insertion enzyme, LipA, which should suffice for lipoic acid biosynthesis. Strain NM57 DELTAlipM is auxotrophic for lipoic acid when grown in minimal medium but grew as well as the wild type strain JH642 in the presence of lipoic acid Bacillus subtilis
physiological function LipM is required for modification of lipoyl domains by the biosynthetic pathway, it is responsible for octanoyl transfer in vivo requirement for LipL. But LipM is only required for the endogenous lipoylation pathway, whereas LipL also plays a role in lipoic acid scavenging Bacillus subtilis