Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli K-12 MG1655 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
F87C | binding pocket mutation | Escherichia coli |
F87C/H244A | H244 plays a key role in stabilizing the oxyanion generated in the condensation reaction through hydrogen bonding | Escherichia coli |
F87I/H244A | H244 plays a key role in stabilizing the oxyanion generated in the condensation reaction through hydrogen bonding | Escherichia coli |
F87S | binding pocket mutation | Escherichia coli |
F87S/N274A | N274 plays a key role in stabilizing the oxyanion generated in the condensation reaction through hydrogen bonding | Escherichia coli |
F87T | binding pocket mutation | Escherichia coli |
F87T/H244A | H244 plays a key role in stabilizing the oxyanion generated in the condensation reaction through hydrogen bonding | Escherichia coli |
H244A | H244 plays a key role in stabilizing the oxyanion generated in the condensation reaction through hydrogen bonding, in the mutant stabilization is reduced inhibiting the condensation reaction, leading to an increase in transacylase activity, allowing larger substrates to be subjected to acyl-ACP to acyl-CoA transacylation | Escherichia coli |
H244A/N274A | N274 and H244 play key roles in stabilizing the oxyanion generated in the condensation reaction through hydrogen bonding | Escherichia coli |
N274A | N274 plays a key role in stabilizing the oxyanion generated in the condensation reaction through hydrogen bonding | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-oxoacyl-ACP + acetyl-CoA | - |
Escherichia coli | acetyl-ACP + 3-ketoacyl-CoA | - |
? | |
acetoacetyl-ACP + acetyl-CoA | - |
Escherichia coli | acetyl-ACP + acetoacetyl-CoA | - |
? | |
malonyl-ACP + acetyl-CoA | - |
Escherichia coli | CO2 + CoA + acetoacetyl-ACP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-ketoacyl-ACP synthase III | - |
Escherichia coli |