Cloned (Comment) | Organism |
---|---|
expression of the His-tagged enzyme in strain BL21(DE3) | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acyl-[acyl-carrier protein] | mixed inhibition type, the enzyme is a target for fatty acid synthesis regulation by acyl-[acyl-carrier-protein], inhibitory potency increases with increasing chain length between C12 and C20 | Escherichia coli | |
additional information | no inhibition by non-esterified [acyl-carrier-protein] | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
malonyl-[acyl-carrier protein] | pH 7.0, 37°C, recombinant enzyme | Escherichia coli | |
0.04 | - |
acetyl-CoA | pH 7.0, 37°C, recombinant enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + malonyl-[acyl-carrier protein] | Escherichia coli | initial condensation reaction to start fatty acid biosynthesis by the type II fatty acid synthase, the enzyme is a target for fatty acid synthesis regulation by acyl-[acyl-carrier-protein] | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene fabH | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from strain BL21(DE3) | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.004 | - |
purified His-tagged recombinant enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + malonyl-[acyl-carrier protein] | initial condensation reaction to start fatty acid biosynthesis by the type II fatty acid synthase, the enzyme is a target for fatty acid synthesis regulation by acyl-[acyl-carrier-protein] | Escherichia coli | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
acetyl-CoA + malonyl-[acyl-carrier protein] | acetyl-CoA and propionyl-CoA are the preferred primers | Escherichia coli | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
butyryl-CoA + malonyl-[acyl-carrier protein] | - |
Escherichia coli | 3-oxo-hexanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
additional information | no activity with hexanoyl-CoA or octanoyl-CoA | Escherichia coli | ? | - |
? | |
propionyl-CoA + malonyl-[acyl-carrier protein] | acetyl-CoA and propionyl-CoA are the preferred primers | Escherichia coli | 3-oxo-valeryl-[acyl-carrier protein] + CoA + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoacyl-acyl carrier protein synthase III | - |
Escherichia coli |
FabH | - |
Escherichia coli |
[acyl-carrier-protein] synthase III | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Escherichia coli |