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Literature summary for 2.3.1.180 extracted from

  • Heath, R.J.; Rock, C.O.
    Inhibition of beta-ketoacyl-acyl carrier protein synthase III (FabH) by acyl-acyl carrier protein in Escherichia coli (1996), J. Biol. Chem., 271, 10996-11000.
    View publication on PubMed
Search for more literature for 2.3.1.180

Cloned(Commentary)

Cloned (Comment) Organism
expression of the His-tagged enzyme in strain BL21(DE3) Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
acyl-[acyl-carrier protein] mixed inhibition type, the enzyme is a target for fatty acid synthesis regulation by acyl-[acyl-carrier-protein], inhibitory potency increases with increasing chain length between C12 and C20 Escherichia coli
additional information no inhibition by non-esterified [acyl-carrier-protein] Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 malonyl-[acyl-carrier protein] pH 7.0, 37°C, recombinant enzyme Escherichia coli
0.04
-
 acetyl-CoA pH 7.0, 37°C, recombinant enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + malonyl-[acyl-carrier protein] Escherichia coli initial condensation reaction to start fatty acid biosynthesis by the type II fatty acid synthase, the enzyme is a target for fatty acid synthesis regulation by acyl-[acyl-carrier-protein] acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 gene fabH
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from strain BL21(DE3) Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.004
-
 purified His-tagged recombinant enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + malonyl-[acyl-carrier protein] initial condensation reaction to start fatty acid biosynthesis by the type II fatty acid synthase, the enzyme is a target for fatty acid synthesis regulation by acyl-[acyl-carrier-protein] Escherichia coli acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
acetyl-CoA + malonyl-[acyl-carrier protein] acetyl-CoA and propionyl-CoA are the preferred primers Escherichia coli acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
butyryl-CoA + malonyl-[acyl-carrier protein]
-
 Escherichia coli 3-oxo-hexanoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
additional information no activity with hexanoyl-CoA or octanoyl-CoA Escherichia coli ?
-
 ?
propionyl-CoA + malonyl-[acyl-carrier protein] acetyl-CoA and propionyl-CoA are the preferred primers Escherichia coli 3-oxo-valeryl-[acyl-carrier protein] + CoA + CO2
-
 ?

Synonyms

Synonyms Comment Organism
beta-ketoacyl-acyl carrier protein synthase III
-
 Escherichia coli
FabH
-
 Escherichia coli
[acyl-carrier-protein] synthase III
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics Escherichia coli