Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Cuphea wrightii |
Protein Variants | Comment | Organism |
---|---|---|
C111A | site-directed mutagenesis, the mutant enzyme loses all condensing activity | Cuphea wrightii |
C111S | site-directed mutagenesis, the mutant enzyme loses all condensing activity, but is still able to bind acetyl-CoA and malonyl-ACP and to decarboxylate the latter to acetyl-ACP | Cuphea wrightii |
C111S/H261A | site-directed mutagenesis, the mutant enzyme loses all condensing activity | Cuphea wrightii |
C111S/H261R | site-directed mutagenesis, the mutant enzyme loses all condensing activity, but is still able to bind acetyl-CoA and malonyl-ACP and to decarboxylate the latter to acetyl-ACP | Cuphea wrightii |
H261A | site-directed mutagenesis, the mutant enzyme loses all condensing activity | Cuphea wrightii |
H261R | site-directed mutagenesis, the mutant enzyme shows reduced condensing activity compared to the wild-type enzyme | Cuphea wrightii |
R150A | site-directed mutagenesis, the mutant enzyme shows reduced condensing activity compared to the wild-type enzyme | Cuphea wrightii |
R266A | site-directed mutagenesis, the mutant enzyme shows reduced condensing activity compared to the wild-type enzyme | Cuphea wrightii |
R306A | site-directed mutagenesis, the mutant enzyme shows highly reduced condensing activity compared to the wild-type enzyme | Cuphea wrightii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Cuphea wrightii | |
0.0014 | - |
acetyl-CoA | pH 7.6, 30°C, recombinant His-tagged wild-type enzyme | Cuphea wrightii | |
0.0041 | - |
malonyl-ACP | pH 7.6, 30°C, recombinant His-tagged wild-type enzyme | Cuphea wrightii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + malonyl-[acyl-carrier protein] | Cuphea wrightii | - |
acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cuphea wrightii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) | Cuphea wrightii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + a malonyl-[acyl-carrier protein] = an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | ping pong reaction mechanism, catalysis involves His261, Arg150, and Arg306, determination of substrate binding site | Cuphea wrightii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Cuphea wrightii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.2 | - |
purified recombinant His-tagged mutant R306A | Cuphea wrightii |
0.8 | - |
purified recombinant His-tagged mutant H261R | Cuphea wrightii |
1.9 | - |
purified recombinant His-tagged mutant R150A | Cuphea wrightii |
3.5 | - |
purified recombinant His-tagged mutant R266A | Cuphea wrightii |
7 | - |
purified recombinant His-tagged wild-type enzyme | Cuphea wrightii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Cuphea wrightii | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-oxoacyl-[acyl-carrier-protein] synthase III | - |
Cuphea wrightii |
ACP synthase III | - |
Cuphea wrightii |
fatty acid synthase type II condensing enzyme | - |
Cuphea wrightii |
KAS III | - |
Cuphea wrightii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Cuphea wrightii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Cuphea wrightii |