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Literature summary for 2.3.1.180 extracted from
- Expression, purification, and crystallization of the Escherichia coli selenomethionyl beta-ketoacyl-acyl carrier protein synthase III (2000), Biochem. Biophys. Res. Commun., 270, 100-107.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of selenomethionine-labeled enzyme in strain BL21(DE3) | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant selenomethionine-labeled enzyme, 0.002 ml of 13 mg/ml protein in 20 mM Tris, pH 7.5, 50 mM NaCl, 10 mM DTT, and 4 mM acetyl-CoA, versus 0.002 ml reservoir solution containing 12-14% PEG 4000, 40 mM Bis-Tris propane, pH 7.0, and 80 mM magnesium acetate, overnight, X-ray diffraction structure determination and analysis at 1.9 A resolution | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33516 | - |
2 * 33516, amino acid sequence calculation, 2 * 33889, selenomethionine-labeled recombinant enzyme, mass spectrometry | Escherichia coli |
| 33889 | - |
2 * 33516, amino acid sequence calculation, 2 * 33889, selenomethionine-labeled recombinant enzyme, mass spectrometry | Escherichia coli |
| 65000 | - |
selenomethionine-labeled recombinant enzyme, gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + malonyl-[acyl-carrier protein] | Escherichia coli | - |
acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene fabH | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant selenomethionine-labeled enzyme by ion exchange, hydroxylapatite, and affinity chromatography, and gel filtration | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.9 | - |
purified recombinant enzyme | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Escherichia coli | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| additional information | the enzyme shows also acyl-CoA-[acyl-carrier-protein] transacylase activity | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 33516, amino acid sequence calculation, 2 * 33889, selenomethionine-labeled recombinant enzyme, mass spectrometry | Escherichia coli |
| More | noncovalent homodimer by dynamic light scattering | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACP synthase III | - |
Escherichia coli |
| beta-ketoacyl-acyl carrier protein synthase III | - |
Escherichia coli |
| FabH | - |
Escherichia coli |
| KAS III | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
unfolding of the recombinant selenomethionine-labeld enzyme starts above | Escherichia coli |
| 70 | - |
about, Tm, recombinant selenomethionine-labeld enzyme | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Escherichia coli |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli | isoelectric focusing, recombinant enzyme | - |
5.1 |
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