Cloned (Comment) | Organism |
---|---|
expression of N-terminally His2-tagged enzyme in Escherichia coli | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
15 mg/ml purified enzyme in 20 mM Tris, pH 8.0, 3 mM DTT, 100 mM NaCl, 10% glycerol, X-ray diffraction structure determination and analysis at 2.0 A resolution | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | 0.014 | isovaleryl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
0.013 | 0.021 | hexanoyl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
0.019 | - |
acetyl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
0.02 | 0.022 | butyryl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
0.024 | 0.037 | isobutyryl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
2 * 36000, recombinant enzyme, SDS-PAGE and gel filtration | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + malonyl-[acyl-carrier protein] | Staphylococcus aureus | - |
acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His2-tagged enzyme from Escherichia coli by nickel and Blue Sepharose affinity chromatography, and gel filtration | Staphylococcus aureus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + a malonyl-[acyl-carrier protein] = an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | ping pong reaction mechanism, catalytically important residues are Phe298 and His238 | Staphylococcus aureus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000001 | - |
purified recombinant enzyme, primer substrate acetyl-CoA | Staphylococcus aureus |
0.000003 | - |
purified recombinant enzyme, primer substrate lauroyl-CoA | Staphylococcus aureus |
0.000005 | - |
purified recombinant enzyme, primer substrate palmitoyl-CoA | Staphylococcus aureus |
0.000006 | - |
purified recombinant enzyme, primer substrate 2-methylcrotonyl-CoA | Staphylococcus aureus |
0.000013 | - |
purified recombinant enzyme, primer substrate propionyl-CoA | Staphylococcus aureus |
0.000015 | - |
purified recombinant enzyme, primer substrate isovaleryl-CoA | Staphylococcus aureus |
0.000022 | - |
purified recombinant enzyme, primer substrate butyryl-CoA | Staphylococcus aureus |
0.000024 | - |
purified recombinant enzyme, primer substrate hexanoyl-CoA | Staphylococcus aureus |
0.000031 | - |
purified recombinant enzyme, primer substrate isobutyryl-CoA | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylcrotonyl-CoA + malonyl-[acyl-carrier protein] | - |
Staphylococcus aureus | (4E)-4-methylhex-4-enoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Staphylococcus aureus | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
acetyl-CoA + malonyl-[acyl-carrier protein] | low activity | Staphylococcus aureus | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
butyryl-CoA + malonyl-[acyl-carrier protein] | - |
Staphylococcus aureus | 3-oxo-hexanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
hexanoyl-CoA + malonyl-[acyl-carrier protein] | high activity | Staphylococcus aureus | 3-oxo-octanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
isobutyryl-CoA + malonyl-[acyl-carrier protein] | isobutyryl-CoA is the preferred primer substrate | Staphylococcus aureus | 3-oxo-4-methyl-pentanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
isovaleryl-CoA + malonyl-[acyl-carrier protein] | - |
Staphylococcus aureus | 3-oxo-5-methyl-hexanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
lauroyl-CoA + malonyl-[acyl-carrier protein] | - |
Staphylococcus aureus | 3-oxo-myristoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
additional information | the enzyme possesses a large primer binding pocket determining the substrate specificity | Staphylococcus aureus | ? | - |
? | |
palmitoyl-CoA + malonyl-[acyl-carrier protein] | - |
Staphylococcus aureus | 3-oxo-stearoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
propionyl-CoA + malonyl-[acyl-carrier protein] | - |
Staphylococcus aureus | 3-oxo-valeryl-[acyl-carrier protein] + CoA + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36000, recombinant enzyme, SDS-PAGE and gel filtration | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoacyl-acyl carrier protein synthase III | - |
Staphylococcus aureus |
FabH | - |
Staphylococcus aureus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.13 | - |
acetyl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
3.8 | - |
butyryl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
4.4 | 7.2 | acetyl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
8.1 | - |
hexanoyl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
15.5 | 30.5 | isovaleryl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
20.9 | 41.6 | butyryl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
25.5 | 41.7 | hexanoyl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
28 | 47.1 | isobutyryl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Staphylococcus aureus |