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Literature summary for 2.3.1.180 extracted from
- beta-Ketoacyl-acyl carrier protein synthase III (FabH) is essential for bacterial fatty acid synthesis (2003), J. Biol. Chem., 278, 51494-51503.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of an enzyme-deficient null mutant strain CL112 by in-frame deletion of FabH active site region, the mutant strain requires unsaturated, but not saturated, long-chain fatty acids for growth, and retains about 10% of wild-type acetate incorporation and fatty acid synthesis activity leading to synthesis of branched-chain fatty acids, analysis of fatty acid content of the mutant strain, overview | Lactococcus lactis ssp. lactis |
| additional information | construction of an enzyme-deficient null mutant strain in which the gene encoding the enzyme from Salmonella enterica serovar typhimurium is inserted as a single copy or in two copies, removal of one of the two copies reduces enzyme activity, removal of the single copy of the gene leads to cell death revealing that the enzyme is absolutely essential for viability, overview | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + malonyl-[acyl-carrier protein] | Escherichia coli | - |
acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? |  |
| acetyl-CoA + malonyl-[acyl-carrier protein] | Lactococcus lactis ssp. lactis | the enzyme is essential for fatty acid biosynthesis, especially of short-chain fatty acids, by fatty acid synthase II initiating the elongation of fatty acids | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| acetyl-CoA + malonyl-[acyl-carrier protein] | Lactococcus lactis ssp. lactis IL1403 | the enzyme is essential for fatty acid biosynthesis, especially of short-chain fatty acids, by fatty acid synthase II initiating the elongation of fatty acids | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| additional information | Lactococcus lactis ssp. lactis | the enzyme prefers short-chain CoA primer substrates | ? | - |
? | |
| additional information | Lactococcus lactis ssp. lactis IL1403 | the enzyme prefers short-chain CoA primer substrates | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene fabH | - |
| Lactococcus lactis ssp. lactis | - |
gene fabH | - |
| Lactococcus lactis ssp. lactis IL1403 | - |
gene fabH | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
analysis of fatty acid content of the wild-type strain | Lactococcus lactis ssp. lactis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Escherichia coli | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Lactococcus lactis ssp. lactis | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| acetyl-CoA + malonyl-[acyl-carrier protein] | the enzyme is essential for fatty acid biosynthesis, especially of short-chain fatty acids, by fatty acid synthase II initiating the elongation of fatty acids | Lactococcus lactis ssp. lactis | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Lactococcus lactis ssp. lactis IL1403 | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| acetyl-CoA + malonyl-[acyl-carrier protein] | the enzyme is essential for fatty acid biosynthesis, especially of short-chain fatty acids, by fatty acid synthase II initiating the elongation of fatty acids | Lactococcus lactis ssp. lactis IL1403 | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| additional information | the enzyme prefers short-chain CoA primer substrates | Lactococcus lactis ssp. lactis | ? | - |
? | |
| additional information | the enzyme prefers short-chain CoA primer substrates | Lactococcus lactis ssp. lactis IL1403 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetoacetyl-ACP synthase | - |
Escherichia coli |
| acetoacetyl-ACP synthase | - |
Lactococcus lactis ssp. lactis |
| beta-ketoacyl-acyl carrier protein synthase III | - |
Escherichia coli |
| beta-ketoacyl-acyl carrier protein synthase III | - |
Lactococcus lactis ssp. lactis |
| FabH | - |
Escherichia coli |
| FabH | - |
Lactococcus lactis ssp. lactis |
| KAS III | - |
Escherichia coli |
| KAS III | - |
Lactococcus lactis ssp. lactis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Lactococcus lactis ssp. lactis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Lactococcus lactis ssp. lactis |
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