Literature summary for 2.3.1.180 extracted from

Verwoert, I.I.G.S.; van der Linden, K.H.; Walsh, M.C.; Nijkamp, H.J.J.; Stuitje, A.R.
Modification of Brassica napus seed oil by expression of the Escherichia coli fabH gene, encoding 3-ketoacyl-acyl carrier protein synthase III (1995), Plant Mol. Biol., 27, 875-886.

Search for more literature for 2.3.1.180

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in strain JM101 inhibits bacterial cell growth and alters the membrane fatty acid composition, overexpression of the chimeric enzyme possessing a transit peptide in Brassica napus seeds and cotyledons via Agrobacterium transfection	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of transgenic Brassica napus plants overexpressing the Escherichia coli enzyme in seeds, expression analysis during seed adevelopment	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics	Escherichia coli
0.045	-	acetyl-CoA	pH 7.0, 25°C, recombinant enzyme from Brassica napus seeds	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	gene fabH	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	recombinant enzyme in Brassica napus seeds	Escherichia coli

Synonyms

Synonyms	Comment	Organism
3-ketoacyl-acyl carrier protein synthase III	-	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)