Literature summary for 2.3.1.177 extracted from

Liu, B.; Raeth, T.; Beuerle, T.; Beerhues, L.
A novel 4-hydroxycoumarin biosynthetic pathway (2010), Plant Mol. Biol., 72, 17-25.

Search for more literature for 2.3.1.177

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli as N-terminally 6His-tagged protein	Sorbus aucuparia

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0007	-	benzoyl-CoA	-	Sorbus aucuparia
0.0008	-	2-hydroxybenzoyl-CoA	-	Sorbus aucuparia
0.0014	-	benzoyl-CoA	-	Sorbus aucuparia
0.0016	-	benzoyl-CoA	-	Sorbus aucuparia
0.0023	-	2-hydroxybenzoyl-CoA	-	Sorbus aucuparia
0.0032	-	2-hydroxybenzoyl-CoA	-	Sorbus aucuparia
0.0062	-	malonyl-CoA	-	Sorbus aucuparia
0.01	-	malonyl-CoA	-	Sorbus aucuparia
0.0101	-	malonyl-CoA	-	Sorbus aucuparia

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
43000	-	-	Sorbus aucuparia
43100	-	-	Sorbus aucuparia

Organism

Organism	UniProt	Comment	Textmining
Sorbus aucuparia	D2DRC4	-	-
Sorbus aucuparia	D2DRC5	-	-
Sorbus aucuparia	Q27Z07	-	-

Purification (Commentary)

Purification (Comment)	Organism
purified by affinity chromatography on a nickel-nitrilotriacetic acid (Ni-NTA) agarose matrix	Sorbus aucuparia

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-hydroxybenzoyl-CoA + malonyl-CoA	-	Sorbus aucuparia	4-hydroxycoumarin + CoA + CO2	-	?
malonyl-CoA + benzoyl-CoA	-	Sorbus aucuparia	CoA + 3,5-dihydroxybiphenyl + CO2	-	?

Synonyms

Synonyms	Comment	Organism
biphenyl synthase	enzyme belongs to the type III polyketide synthases	Sorbus aucuparia
BIS	enzyme belongs to the type III polyketide synthases	Sorbus aucuparia
BIS1	-	Sorbus aucuparia
BIS2	enzyme belongs to the type III polyketide synthases, prefering 2-hydroxybenzoyl-CoA as a starter molecule instead of benzoyl-CoA	Sorbus aucuparia
BIS3	enzyme belongs to the type III polyketide synthases, prefering 2-hydroxybenzoyl-CoA as a starter molecule instead of benzoyl-CoA	Sorbus aucuparia

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
35	-	-	Sorbus aucuparia

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0045	-	2-hydroxybenzoyl-CoA	-	Sorbus aucuparia
0.00717	-	benzoyl-CoA	-	Sorbus aucuparia
0.01483	-	benzoyl-CoA	-	Sorbus aucuparia
0.017	-	benzoyl-CoA	-	Sorbus aucuparia
0.02933	-	2-hydroxybenzoyl-CoA	-	Sorbus aucuparia
0.037	-	2-hydroxybenzoyl-CoA	-	Sorbus aucuparia

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	7	-	Sorbus aucuparia

pI Value

Organism	Comment	pI Value Maximum	pI Value
Sorbus aucuparia	-	-	5.45
Sorbus aucuparia	-	-	6.42

General Information

General Information	Comment	Organism
metabolism	BIS is the key enzyme of biphenyl metabolism, producing for instance 3,5-dihydroxybiphenyl which is the precursor of the phytoalexins of the Maloideae.	Sorbus aucuparia

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
5.625	-	2-hydroxybenzoyl-CoA	-	Sorbus aucuparia
10.18	-	benzoyl-CoA	-	Sorbus aucuparia
10.63	-	benzoyl-CoA	-	Sorbus aucuparia
10.99	-	benzoyl-CoA	-	Sorbus aucuparia
11.49	-	2-hydroxybenzoyl-CoA	-	Sorbus aucuparia
12.72	-	2-hydroxybenzoyl-CoA	-	Sorbus aucuparia

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Release 2023.1 (January 2023)