Organism | UniProt | Comment | Textmining |
---|---|---|---|
synthetic construct | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
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acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein] | investigation of putative intermediate states from the catalytic cycle by hybrid density functional theory. A mononuclear mechanism in which CO and CH3 bind the proximal nickel is favored over the binuclear mechanism in which CO and CH3 bind the proximal and distal nickel ions resp. The formation of a disulfide bond in the active site could provide the two electrons necessary for the reaction if methylation occurs simultaneously. The crystallographic closed form of the active site needs to open to accomodate ligands in the equatorial state | synthetic construct |