Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.166 extracted from

  • Walker, K.; Croteau, R.
    Taxol biosynthesis: molecular cloning of a benzoyl-CoA:taxane 2alpha-O-benzoyltransferase cDNA from Taxus and functional expression in Escherichia coli (2000), Proc. Natl. Acad. Sci. USA, 97, 13591-13596.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 Taxus cuspidata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
benzoyl-CoA
-
Taxus cuspidata
0.64
-
2-debenzoyl-7,13-diacetylbaccatin III
-
Taxus cuspidata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
ca. x * 50000, SDS-PAGE Taxus cuspidata
50090
-
calculated from sequence of cDNA Taxus cuspidata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzoyl-CoA + 10-deacetyl-2-debenzoylbaccatin III Taxus cuspidata
-
CoA + 10-deacetylbaccatin III
-
?

Organism

Organism UniProt Comment Textmining
Taxus cuspidata Q9FPW3
-
-

Purification (Commentary)

Purification (Comment) Organism
partially Taxus cuspidata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-CoA + 10-deacetyl-2-debenzoylbaccatin III
-
Taxus cuspidata CoA + 10-deacetylbaccatin III
-
?
benzoyl-CoA + 2-debenzoyl-7,13-diacetylbaccatin III 2-debenzoyl-7,13-diacetylbaccatin III is a semisynthetic substrate Taxus cuspidata CoA + 7,13-diacetylbaccatin III
-
?
additional information enzyme does not benzoylate the 1beta-, 7beta-, 10beta- or 13alpha-hydroxyl groups of 10-deacetylbaccatin III, nor does it benzoylate the 2alpha- or 5alpha-hydroxyl groups of taxa-4(20),11(12)-dien-2alpha,5alpha-diol, acetyl-CoA no cosubstrate Taxus cuspidata ?
-
?

Subunits

Subunits Comment Organism
? ca. x * 50000, SDS-PAGE Taxus cuspidata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
31
-
enzyme assay Taxus cuspidata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Taxus cuspidata

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.9
-
Taxus cuspidata