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Literature summary for 2.3.1.165 extracted from

  • Parascandolo, J.S.; Havemann, J.; Potter, H.K.; Huang, F.; Riva, E.; Connolly, J.; Wilkening, I.; Song, L.; Leadlay, P.F.; Tosin, M.
    Insights into 6-methylsalicylic acid bio-assembly by using chemical probes (2016), Angew. Chem. Int. Ed. Engl., 55, 3463-3467.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Penicillium griseofulvum

Protein Variants

Protein Variants Comment Organism
H958A mutation in the THID active site Penicillium griseofulvum

Organism

Organism UniProt Comment Textmining
Penicillium griseofulvum P22367
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetoacetyl-CoA + 3 malonyl-CoA + NADPH + H+
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 Penicillium griseofulvum 6-methyl triacetic acid lactone + CoA + NADP+
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 ?
acetyl-CoA + 3 malonyl-CoA + NADPH + H+
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 Penicillium griseofulvum 6-methylsalicylate + 4 CoA + 3 CO2 + NADP+ + H2O
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additional information no enzymatic dehydration takes place at a triketide stage of 6-MSA assembly, so loss of water must occur at the tetraketide stage. The enzyme is able to accept malonate surrogates of different chain lengths and bearing various functionalities (including alkyne and fluorine moieties) at different positions and for every round of chain extension, and also to generate novel pentaketide products Penicillium griseofulvum ?
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