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Literature summary for 2.3.1.165 extracted from

  • Wattanachaisaereekul, S.; Lantz, A.E.; Nielsen, M.L.; Andresson, O.S.; Nielsen, J.
    Optimization of heterologous production of the polyketide 6-MSA in Saccharomyces cerevisiae (2006), Biotechnol. Bioeng., 97, 893-900.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information in order to activate 6-MSAS a 4'-phosphopantetheinyl transferase, PPTase, is required, therefor a Bacillus subtilis or a Aspergillus nidulans PPTase is coexpressed in yeast Penicillium griseofulvum

Application

Application Comment Organism
biotechnology functional heterologous expression of enzyme requires the presence of a 4’-phosphoantetheinyl transferase for activation. Coexpression of both enzymes results in production of 6-methylsalicylic acid in good yields Penicillium griseofulvum
biotechnology polyketides are a group of natural products that have gained much interest due to their use as antibiotics, cholesterol lowering agents, immunosuppressors and other drugs, it is therefor of general interest to transfer polyketide synthase genes into heterologous hosts that can overproduce the corresponding polyketides Penicillium griseofulvum

Cloned(Commentary)

Cloned (Comment) Organism
for expression in Saccharomyces cerevisiae Penicillium griseofulvum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + malonyl-CoA + NADPH + H+ Penicillium griseofulvum
-
6-methylsalicylate + CoA + CO2 + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Penicillium griseofulvum
-
expression in Saccharomyces cerevisiae
-
Penicillium griseofulvum P22367
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + malonyl-CoA + NADPH + H+
-
Penicillium griseofulvum 6-methylsalicylate + CoA + CO2 + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
6-methylsalicylic-acid synthase
-
Penicillium griseofulvum
6-MSAS
-
Penicillium griseofulvum