Cloned (Comment) | Organism |
---|---|
gene penDE | Penicillium chrysogenum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
pro-enzyme | Penicillium chrysogenum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenylacetyl-CoA + isopenicillin N + H2O | Penicillium chrysogenum | - |
CoA + penicillin G + L-2-aminohexanedioate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium chrysogenum | P15802 | gene penDE | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the proenzyme is processed autocatalytically to generate two polypeptides forming alpha-subunit and beta-subunit | Penicillium chrysogenum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenylacetyl-CoA + isopenicillin N + H2O | - |
Penicillium chrysogenum | CoA + penicillin G + L-2-aminohexanedioate | - |
? | |
phenylacetyl-CoA + isopenicillin N + H2O | IAT substitutes the alpha-aminoadipic acid side chain of isopenicillin N by a phenylacetic acid phenolate group from phenylacetyl-CoA | Penicillium chrysogenum | CoA + penicillin G + L-2-aminohexanedioate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | three-dimensional structure determined by homology modeling and molecular docking in different snapshots, and refined by molecular dynamic simulations, detailed overview. The gene for IAT, penDE, encodes a 357 residue proenzyme with a molecular weight of 40 kDa, which is processed autocatalytically to generate two polypeptides of 102 residues as alpha-subunit and 255 residues as beta-subunit | Penicillium chrysogenum |
Synonyms | Comment | Organism |
---|---|---|
acyl coA:isopenicillin N-acyltransferase | - |
Penicillium chrysogenum |
IAT | - |
Penicillium chrysogenum |
General Information | Comment | Organism |
---|---|---|
metabolism | in the last step of penicillin biosynthesis, acyl-CoA:isopenicillin N acyltransferase catalyzes the conversion of isopenicillin N to penicillin G | Penicillium chrysogenum |
additional information | substrate binding structure determined by homology modeling and molecular docking in different snapshots, and refined by molecular dynamic simulations. A site located in the region generated by beta1, beta2 and beta5 strands, which forms part of the central structure of beta-subunit, as the potential binding site of isopenicillin N, which establishes interactions with Cys103, Leu169, Gln172, Asp264, and Arg310, detailed overview | Penicillium chrysogenum |