KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
(4E,6E)-3-oxoocta-4,6-dienoyl-N-acetylcysteamine | pH not specified in the publication, temperature not specified in the publication | Aspergillus terreus | |
2 | - |
3-oxohexanoyl-N-acetylcysteamine | pH not specified in the publication, temperature not specified in the publication | Aspergillus terreus | |
5.2 | - |
3-oxooctanoyl-N-acetylcysteamine | pH not specified in the publication, temperature not specified in the publication | Aspergillus terreus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4E,6E)-2-methyl-3-oxoocta-4,6-dienoyl-S-ACP + NADPH + H+ | Aspergillus terreus | reaction of ketoreductase subunit, natural substrate. ACP = acyl-carrier protein | (4E,6E)-2-methyl-3-hydroxyocta-4,6-dienoyl-S-ACP + NADP+ | - |
? | |
(4E,6E)-3-oxoocta-4,6-dienoyl-S-ACP + S-adenosyl-L-methionine | Aspergillus terreus | reaction of methyltransferase subunit, natural substrate. ACP = acyl-carrier protein | (4E,6E)-2-methyl-3-oxoocta-4,6-dienoyl-S-ACP + S-adenosyl-L-homocysteine | - |
? | |
2-methyl-3-oxohexanoyl-N-acetylcysteamine + NADPH + H+ | Aspergillus terreus | reaction of ketoreductase subunit, natural substrate | 3-hydroxy-2-methylhexanoyl-N-acetylcysteamine + NADP+ | - |
? | |
3-oxohexanoyl-N-acetylcysteamine + NADPH + H+ | Aspergillus terreus | reaction of ketoreductase subunit, natural substrate | 3-hydroxyhexanoyl-N-acetylcysteamine + NADP+ | - |
? | |
additional information | Aspergillus terreus | the methyltransferase domain displays methylation activity toward different beta-ketoacyl groups, it is exceptionally selective toward its naturally programmed beta-keto-dienyltetraketide substrate with respect to both chain length and functionalization. The ketoreductase domain displays broader substrate specificity toward different beta-ketoacyl groups | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus terreus | Q9Y8A5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4E,6E)-2-methyl-3-oxoocta-4,6-dienoyl-S-ACP + NADPH + H+ | reaction of ketoreductase subunit, natural substrate. ACP = acyl-carrier protein | Aspergillus terreus | (4E,6E)-2-methyl-3-hydroxyocta-4,6-dienoyl-S-ACP + NADP+ | - |
? | |
(4E,6E)-3-oxoocta-4,6-dienoyl-N-acetylcysteamine + S-adenosyl-L-methionine | reaction of methyltransferase subunit, natural substrate analogue. ACP = acyl-carrier protein | Aspergillus terreus | (4E,6E)-2-methyl-3-oxoocta-4,6-dienoyl-N-acetylcysteamine + S-adenosyl-L-homocysteine | - |
? | |
(4E,6E)-3-oxoocta-4,6-dienoyl-S-ACP + S-adenosyl-L-methionine | reaction of methyltransferase subunit, natural substrate. ACP = acyl-carrier protein | Aspergillus terreus | (4E,6E)-2-methyl-3-oxoocta-4,6-dienoyl-S-ACP + S-adenosyl-L-homocysteine | - |
? | |
2-methyl-3-oxohexanoyl-N-acetylcysteamine + NADPH + H+ | reaction of ketoreductase subunit, natural substrate | Aspergillus terreus | 3-hydroxy-2-methylhexanoyl-N-acetylcysteamine + NADP+ | - |
? | |
3-oxohexanoyl-N-acetylcysteamine + NADPH + H+ | reaction of ketoreductase subunit, natural substrate | Aspergillus terreus | 3-hydroxyhexanoyl-N-acetylcysteamine + NADP+ | - |
? | |
3-oxooctanoyl-N-acetylcysteamine + S-adenosyl-L-methionine | reaction of methyltransferase subunit, artificial substrate. 0.3% of the activity with (4E,6E)-3-oxoocta-4,6-dienoyl-N-acetylcysteamine | Aspergillus terreus | 2-methyl-3-oxooctanoyl-N-acetylcysteamine + S-adenosyl-L-homocysteine | - |
? | |
additional information | the methyltransferase domain displays methylation activity toward different beta-ketoacyl groups, it is exceptionally selective toward its naturally programmed beta-keto-dienyltetraketide substrate with respect to both chain length and functionalization. The ketoreductase domain displays broader substrate specificity toward different beta-ketoacyl groups | Aspergillus terreus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.085 | - |
2-methyl-3-oxohexanoyl-N-acetylcysteamine | pH not specified in the publication, temperature not specified in the publication | Aspergillus terreus | |
0.32 | - |
3-oxooctanoyl-N-acetylcysteamine | pH not specified in the publication, temperature not specified in the publication | Aspergillus terreus | |
0.57 | - |
3-oxohexanoyl-N-acetylcysteamine | pH not specified in the publication, temperature not specified in the publication | Aspergillus terreus | |
3.25 | - |
(4E,6E)-3-oxoocta-4,6-dienoyl-N-acetylcysteamine | pH not specified in the publication, temperature not specified in the publication | Aspergillus terreus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme utilizes an oxidized quinone as cofactor | Aspergillus terreus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
3-oxooctanoyl-N-acetylcysteamine | pH not specified in the publication, temperature not specified in the publication | Aspergillus terreus | |
0.31 | - |
3-oxohexanoyl-N-acetylcysteamine | pH not specified in the publication, temperature not specified in the publication | Aspergillus terreus | |
18.93 | - |
(4E,6E)-3-oxoocta-4,6-dienoyl-N-acetylcysteamine | pH not specified in the publication, temperature not specified in the publication | Aspergillus terreus |