KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0075 | - |
gardneral | pH 7.0, 30°C | Rauvolfia serpentina x Rhazya stricta | |
0.057 | - |
acetyl-CoA | pH 7.0, 30°C | Rauvolfia serpentina x Rhazya stricta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
about, gel filtration | Rauvolfia serpentina x Rhazya stricta |
50000 | - |
1 * 50000, about, SDS-PAGE | Rauvolfia serpentina x Rhazya stricta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 16-epivellosimine | Rauvolfia serpentina x Rhazya stricta | the enzyme is involved in a crucial step of antiarrhythmic drug ajmaline biosynthesis, pathway overview | CoA + vinorine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rauvolfia serpentina x Rhazya stricta | - |
hybrid | - |
Purification (Comment) | Organism |
---|---|
991fold from cell suspension culture by anion exchange, hydrophobic interaction, and hydroxyapatite chromatography, and gel filtration | Rauvolfia serpentina x Rhazya stricta |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | hybrid of Rauvolfia serpentina and Rhazya stricta | Rauvolfia serpentina x Rhazya stricta | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
99.1 | - |
purified enzyme | Rauvolfia serpentina x Rhazya stricta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 16-epivellosimine | the enzyme is involved in a crucial step of antiarrhythmic drug ajmaline biosynthesis, pathway overview | Rauvolfia serpentina x Rhazya stricta | CoA + vinorine | - |
? | |
acetyl-CoA + 16-epivellosimine | the forward reaction is preferred | Rauvolfia serpentina x Rhazya stricta | CoA + vinorine | - |
r | |
acetyl-CoA + gardneral | sarpagan-type alkaloid, the forward reaction is preferred | Rauvolfia serpentina x Rhazya stricta | CoA + 11-methoxyvinorine | ajmalin-type alkaloid | r | |
additional information | the acetylation is important for stabilization of the immediate indolenine 17-deacetyl-vinorine, which would otherwise be spontaneously re-transformed | Rauvolfia serpentina x Rhazya stricta | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, about, SDS-PAGE | Rauvolfia serpentina x Rhazya stricta |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme probably belongs to the BAHD enzyme super family | Rauvolfia serpentina x Rhazya stricta |
vinorine synthase | - |
Rauvolfia serpentina x Rhazya stricta |
VS | - |
Rauvolfia serpentina x Rhazya stricta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rauvolfia serpentina x Rhazya stricta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Rauvolfia serpentina x Rhazya stricta |