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Literature summary for 2.3.1.16 extracted from

  • Yamashita, H.; Itsuki, A.; Kimoto, M.; Hiemori, M.; Tsuji, H.
    Acetate generation in rat liver mitochondria: acetyl-CoA hydrolase activity is demonstrated by 3-ketoacyl-CoA thiolase (2006), Biochim. Biophys. Acta, 1761, 17-23.
    View publication on PubMed
Search for more literature for 2.3.1.16

Activating Compound

Activating Compound Comment Organism Structure
additional information di-(2-ethylhexyl)phthalate induces the enzyme Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
acetoacetyl-CoA competitive inhibition of the acetyl-CoA hydrolase activity Rattus norvegicus
acetyl-CoA competitive inhibition of the 3-ketoacyl-CoA thiolase activity Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Rattus norvegicus
0.0114
-
 acetoacetyl-CoA pH 7.4 Rattus norvegicus
0.71
-
 acetyl-CoA pH 7.4 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + H2O Rattus norvegicus 3-ketoacyl-CoA thiolase activity acetate + CoA
-
 ?
additional information Rattus norvegicus the enzyme is involved in fatty acid beta-oxidation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 27.2fold from liver mitochondria preparation by 5 steps of ion exchange and hydrophobic interaction chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brown adipose tissue di-(2-ethylhexyl)phthalate-inducible acetyl-CoA hydrolase activity Rattus norvegicus
-
heart di-(2-ethylhexyl)phthalate-inducible acetyl-CoA hydrolase activity Rattus norvegicus
-
intestine di-(2-ethylhexyl)phthalate-inducible acetyl-CoA hydrolase activity Rattus norvegicus
-
kidney di-(2-ethylhexyl)phthalate-inducible acetyl-CoA hydrolase activity Rattus norvegicus
-
liver di-(2-ethylhexyl)phthalate-inducible acetyl-CoA hydrolase activity Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.543
-
 purified enzyme, acetyl-CoA hydrolase activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O 3-ketoacyl-CoA thiolase activity Rattus norvegicus acetate + CoA
-
 ?
acetyl-CoA + H2O best acyl-CoA substrate, acetyl-CoA hydrolase activity Rattus norvegicus acetate + CoA
-
 ?
acetyl-CoA + H2O preferred substrate, 3-ketoacyl-CoA thiolase activity Rattus norvegicus acetate + CoA
-
 ?
butyryl-CoA + H2O acetyl-CoA hydrolase activity Rattus norvegicus butanoate + CoA
-
 ?
dodecanoyl-CoA + H2O acetyl-CoA hydrolase activity Rattus norvegicus dodecanoate + CoA
-
 ?
hexanoyl-CoA + H2O acetyl-CoA hydrolase activity Rattus norvegicus hexanoate + CoA
-
 ?
additional information the enzyme is involved in fatty acid beta-oxidation Rattus norvegicus ?
-
 ?
additional information analysis of the substrate chain length specificity of the enzyme, high preference for acetyl-CoA Rattus norvegicus ?
-
 ?
palmitoyl-CoA + H2O acetyl-CoA hydrolase activity Rattus norvegicus palmitate + CoA
-
 ?
propionyl-CoA + H2O acetyl-CoA hydrolase activity Rattus norvegicus propionate + CoA
-
 ?

Synonyms

Synonyms Comment Organism
3-ketoacyl-CoA thiolase
-
 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0022
-
 acetoacetyl-CoA pH 7.4, competitive versus acetyl-CoA hydrolysis Rattus norvegicus
0.24
-
 acetyl-CoA pH 7.4, competitive versus 3-ketoacyl-CoA thiolase activity Rattus norvegicus