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Literature summary for 2.3.1.159 extracted from

  • Baumert, A.; Maier, W.; Gröger, D.; Deutzmann, R.
    Purification and properties of acridone synthase from cell suspension cultures of Ruta graveolens L. (1994), Z. Naturforsch. C, 49, 26-32.
    View publication on PubMed
Search for more literature for 2.3.1.159

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide 0.5 mM: 95% inhibition Ruta graveolens
N-ethylmaleimide 1 mM: 100% inhibition Ruta graveolens
p-chloromercuribenzoate 0.5 mM: 95% inhibition Ruta graveolens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0106
-
 N-methylanthraniloyl-CoA partially purified enzyme Ruta graveolens
0.0328
-
 malonyl-CoA partially purified enzyme Ruta graveolens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid sequence determination Ruta graveolens
40000
-
 2 * 40000, SDS-PAGE Ruta graveolens
69000
-
 gel filtration Ruta graveolens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malonyl-CoA + N-methylanthraniloyl-CoA Ruta graveolens
-
 CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Ruta graveolens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ruta graveolens

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Ruta graveolens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.035
-
 purified enzyme Ruta graveolens

Storage Stability

Storage Stability Organism
-70°C, partially purified, stable for at least 4 weeks Ruta graveolens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + N-methylanthraniloyl-CoA
-
 Ruta graveolens CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Ruta graveolens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
 assay at Ruta graveolens