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Literature summary for 2.3.1.158 extracted from

  • van Erp, H.; Bates, P.D.; Burgal, J.; Shockey, J.; Browse, J.
    Castor phospholipid:diacylglycerol acyltransferase facilitates efficient metabolism of hydroxy fatty acids in transgenic Arabidopsis (2011), Plant Physiol., 155, 683-693.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis the enzyme is useful for production of hydroxylated fatty acids and furtheron polyesters, biodiesel, and lubricants in transgenic plants expressing PDAT thereby avoiding the toxicity of castor bean seeds Ricinus communis

Cloned(Commentary)

Cloned (Comment) Organism
isozyme PDAT2 and PDAT1A, cloning from cDNA library, expression in Arabidopsis thaliana, coexpression of PDAT1A with Ricinus communis hydroxylated fatty acid hydroxylase, RcFAH, in Arabidopsis thaliana causes a reduction of fatty acids by 73% compared to wild-type plants, isozyme PDAT2 is also functional but less active than isozyme PDAT1A, method optimization, phenotypes, overview Ricinus communis

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Ricinus communis
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipid + 1,2-diacyl-sn-glycerol Ricinus communis
-
lysophospholipid + triacylglycerol
-
?

Organism

Organism UniProt Comment Textmining
Ricinus communis
-
three genes encoding isozymes PDAT1A and PDAT2, and PDAT1B
-

Source Tissue

Source Tissue Comment Organism Textmining
endosperm both PDAT1A and PDAT2 are expressed in the developing endosperm, with PDAT2 showing double the transcript levels of PDAT1A. No expression of RcPDAT1B in castor endosperm Ricinus communis
-
seed
-
Ricinus communis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phospholipid + 1,2-diacyl-sn-glycerol
-
Ricinus communis lysophospholipid + triacylglycerol
-
?

Synonyms

Synonyms Comment Organism
PDAT
-
Ricinus communis
PDAT1A
-
Ricinus communis