Literature summary for 2.3.1.157 extracted from

Kostrewa, D.; D'Arcy, A.; Takacs, B.; Kamber, M.
Crystal structures of Streptococcus pneumoniae N-acetylglucosamine-1-phosphate uridyltransferase, GlmU, in apo form at 2.33 A resolution and in complex with UDP-N-acetylglucosamine and Mg(2+) at 1.96 A resolution (2001), J. Mol. Biol., 305, 279-289.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
vapor-diffusion method, crystal structure of the enzyme in apo form at 2.33 A resolution, and in complex with UDP-N-acetyl glucosamine and the essential cofactor Mg2+ at 1.96 A resolution. In the crystal, the enzyme forms exact trimers, mainly through contacts between left-handed beta-sheet helix domains. UDP-N-acetylglucosamine and Mg2+ are bound at the uridyltransferase active site, which is in a closed form	Streptococcus pneumoniae

Crystallization (Comment)

Organism

vapor-diffusion method, crystal structure of the enzyme in apo form at 2.33 A resolution, and in complex with UDP-N-acetyl glucosamine and the essential cofactor Mg2+ at 1.96 A resolution. In the crystal, the enzyme forms exact trimers, mainly through contacts between left-handed beta-sheet helix domains. UDP-N-acetylglucosamine and Mg2+ are bound at the uridyltransferase active site, which is in a closed form

Streptococcus pneumoniae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glucosamine 1-phosphate + acetyl-CoA	Streptococcus pneumoniae	the bifunctional enzyme also possesses the activity of EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase and performs the last two steps in the synthesis of UDP-N-acetylglucosamine, which is an essential precursor in both the peptidoglycan and the lipopolysaccharide metabolic pathway	N-acetyl-D-glucosamine 1-phosphate + CoA	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pneumoniae	Q97R46	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucosamine 1-phosphate + acetyl-CoA	the bifunctional enzyme also possesses the activity of EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase and performs the last two steps in the synthesis of UDP-N-acetylglucosamine, which is an essential precursor in both the peptidoglycan and the lipopolysaccharide metabolic pathway	Streptococcus pneumoniae	N-acetyl-D-glucosamine 1-phosphate + CoA	-	?
D-glucosamine 1-phosphate + acetyl-CoA	a mechanism is proposed in which the activation energy of the double negatively charged phosphorane transition state is lowered by charge compensation of Mg2+ and the side-chain of Lys22	Streptococcus pneumoniae	N-acetyl-D-glucosamine 1-phosphate + CoA	-	?

Synonyms

Synonyms	Comment	Organism
GlmU	bifunctional enzyme also possesses the activity of EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase	Streptococcus pneumoniae
N-acetylglucosamine-1-phosphate uridyltransferase	bifunctional enzyme also possesses the activity of EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase	Streptococcus pneumoniae