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Literature summary for 2.3.1.156 extracted from

  • Paniego, N.B.; Zuurbier, K.W.M.; Fung, S.Y.; Van der Heijden, R.; Scheffer, J.J.C.; Verpoorte, R.
    Phlorisovalerophenone synthase, a novel polyketide synthase from hop (Humulus lupulus L.) cones (1999), Eur. J. Biochem., 262, 612-616.
    View publication on PubMed
Search for more literature for 2.3.1.156

Application

Application Comment Organism
nutrition cones of the hop plant used in the beer-brewing process Humulus lupulus

Cloned(Commentary)

Cloned (Comment) Organism
characterization of the VPS cDNA clone Humulus lupulus

General Stability

General Stability Organism
low stability Humulus lupulus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 isovaleryl-CoA
-
 Humulus lupulus
0.01
-
 isobutyryl-CoA
-
 Humulus lupulus
0.033
-
 malonyl-CoA
-
 Humulus lupulus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 homodimer, 2 * 45000, SDS-PAGE Humulus lupulus
110000
-
 native PAGE of purified VPS Humulus lupulus

Organism

Organism UniProt Comment Textmining
Humulus lupulus
-
 hop
-

Purification (Commentary)

Purification (Comment) Organism
-
 Humulus lupulus

Reaction

Reaction Comment Organism Reaction ID
isovaleryl-CoA + 3 malonyl-CoA = 4 CoA + 3 CO2 + phlorisovalerophenone Closely related to EC 2.3.1.74, chalcone synthase. The product, 3-methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one, is chloroisovalerophenone. Also acts on isobutyryl-CoA as substrate to give phlorisobutyrophenone. The products are intermediates in the biosynthesis of the bitter alpha-acids in hops, Humulus lupulus Humulus lupulus
a

Source Tissue

Source Tissue Comment Organism Textmining
cone glandular hairs Humulus lupulus
-
inflorescence glands Humulus lupulus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isobutyryl-CoA + 3 malonyl-CoA
-
 Humulus lupulus phloroisobutyrophenone + 3 CO2 + 4 CoA
-
 ?
isovaleryl-CoA + malonyl-CoA
-
 Humulus lupulus phloroisovalerophenone + CO2 + CoA
-
 ?

Subunits

Subunits Comment Organism
dimer homodimer, 2 * 45000, SDS-PAGE Humulus lupulus

Synonyms

Synonyms Comment Organism
VPS
-
 Humulus lupulus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Humulus lupulus