Cloned (Comment) | Organism |
---|---|
gene MS33 or SA1, map-based cloning and gene structure of SA1/MS33, DNA and amino acid sequence determination and analysis, phylogenetic analysis of MS33, quantitative PCR enzyme expression analysis | Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant ms33 is allelic to mutant sa1, male-sterile mutant sa1 phenotype compared to wild-type, overview | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | Zea mays | - |
CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | A0A1D6F8B0 | MS33 | - |
Zea mays | A0A1D6JQM9 | SA1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
anther | the enzyme is preferentially expressed in tapetal cells during anther development. Strong MS33 expression is detected in the tapetum from pre-meiosis to the binucleate stage | Zea mays | - |
anther | the enzyme is preferentially expressed in tapetal cells during anther development. Strong MS33 expression is detected in the tapetum from pre-meiosis to the binucleate stage. Strong MS33 expression is detected in the tapetum from pre-meiosis to the binucleate stage | Zea mays | - |
leaf | - |
Zea mays | - |
additional information | quantitative PCR enzyme expression analysis, MS33 expression is dramatically stimulated in developing anthers, reaching a peak at the tetrad stage | Zea mays | - |
root | - |
Zea mays | - |
stem | - |
Zea mays | - |
tapetum | - |
Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | - |
Zea mays | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycerol-3-phosphate acyltransferase | - |
Zea mays |
GPAT | - |
Zea mays |
MS33 | - |
Zea mays |
SA1 | - |
Zea mays |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic analysis of MS33 | Zea mays |
malfunction | the enzyme mutant plants show substantial reduction in wax and cutin in ms33 anthers compared to wild-type. The maize male-sterile mutant shrinking anther 1 (sa1), which is allelic to the classic mutant male sterile 33 (ms33), displays defective anther cuticle development and premature microspore degradation. Male-sterile mutant sa1 phenotype compared to wild-type, overview. Female fertility is not affected in sa1, but the microspores of sa1 appear irregular, the anther layers appear collapsed, and the microspores are defective. At the mature pollen grain stage, in wild-type anthers, many pollen grains are present in the anther locule, whereas in sa1 anthers, no pollen grains are observed, and only some residual debris remain. Comparison of cutin and wax compositions of wild-type and mutant enzymes | Zea mays |
physiological function | enzyme MS33 (SA1) may play an essential role in anther cuticle and pollen grain/microspore development by affecting lipid polyester biosynthesis in maize. MS33 affects the expression of genes involved in wax and cutin biosynthesis | Zea mays |