Cloned (Comment) | Organism |
---|---|
into the pGEM12zf+ vector | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial outer membrane | - |
Rattus norvegicus | 5741 | - |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
92000 | - |
determined by SDS-PAGE and Western Blot analysis | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | Rattus norvegicus | - |
CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male wistar rat | - |
Purification (Comment) | Organism |
---|---|
mitochondria and submitochondrial fractions are purified by gradient centrifugation, radiolabeled GPAT1 is synthesized by an in vitro transcription-translation system | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00016 | - |
contact sites obtained from mitochondria, subfraction of crude mitochondria | Rattus norvegicus |
0.00065 | - |
crude mitochondria | Rattus norvegicus |
0.00065 | - |
inner mitochondrial membrane, subfraction of crude mitochondria | Rattus norvegicus |
0.0009 | - |
inner mitochondrial membrane, subfraction of pure mitochondria | Rattus norvegicus |
0.0011 | - |
pure mitochondria | Rattus norvegicus |
0.00148 | - |
pure mitochondria, mitochondrial associated vesicle fraction | Rattus norvegicus |
0.0016 | - |
outer mitochondrial membrane, subfraction of crude mitochondria | Rattus norvegicus |
0.0029 | - |
contact sites obtained from mitochondria, subfraction of pure mitochondria | Rattus norvegicus |
0.0031 | - |
outer mitochondrial membrane, subfraction of pure mitochondria | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | - |
Rattus norvegicus | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycerol-3-P acyltransferase 1 | - |
Rattus norvegicus |
glycerol-3-phosphate acyltransferase-1 | - |
Rattus norvegicus |
GPAT1 | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assasy | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
activity assasy | Rattus norvegicus |