Cloned (Comment) | Organism |
---|---|
PCR amplified mouse testis cDNA is cloned into the TOPO vector and subsequently subcloned into pcDNA3.1 for transient transfection of COS-7 and HEK293 cells | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | - |
Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Mus musculus | 16020 | - |
mitochondrion | - |
Mus musculus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
88800 | - |
calculated molecular mass | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | Mus musculus | - |
CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to determine the translated size of GPAT2, in vitro transcription and translation is performed | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brown adipose tissue | - |
Mus musculus | - |
liver | - |
Mus musculus | - |
testis | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0002 | - |
with empty pcDNA3.1 vector transfected COS-7 cells, N-ethylmaleimide resistant | Mus musculus |
0.0003 | - |
control, untransfected COS-7 cells, N-ethylmaleimide resistant | Mus musculus |
0.0003 | - |
with pcDNA3.1-GPAT2 transfected COS-7 cells, N-ethylmaleimide resistant | Mus musculus |
0.0012 | - |
HEK293 cells | Mus musculus |
0.00149 | - |
transfected HEK293 cells, 18 h after transfection | Mus musculus |
0.00188 | - |
transfected HEK293 cells, 36 h after transfection | Mus musculus |
0.003 | - |
with empty pcDNA3.1 vector transfected COS-7 cells, N-ethylmaleimide sensitive | Mus musculus |
0.0032 | - |
with empty pcDNA3.1 vector transfected COS-7 cells, total | Mus musculus |
0.0033 | - |
control, untransfected COS-7 cells, N-ethylmaleimide sensitive | Mus musculus |
0.0035 | - |
control, untransfected COS-7 cells, total | Mus musculus |
0.0042 | - |
with pcDNA3.1-GPAT2 transfected COS-7 cells, N-ethylmaleimide sensitive | Mus musculus |
0.0044 | - |
with pcDNA3.1-GPAT2 transfected COS-7 cells, total | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | - |
Mus musculus | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycerol-3-phosphate acyltransferase | - |
Mus musculus |
GPAT2 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
activity assay | Mus musculus |