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Literature summary for 2.3.1.15 extracted from

  • Nagle, C.A.; An, J.; Shiota, M.; Torres, T.P.; Cline, G.W.; Liu, Z.X.; Wang, S.; Catlin, R.L.; Shulman, G.I.; Newgard, C.B.; Coleman, R.A.
    Hepatic overexpression of glycerol-sn-3-phosphate acyltransferase 1 in rats causes insulin resistance (2007), J. Biol. Chem., 282, 14807-14815.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.15

Application

Application Comment Organism
medicine major finding of the study is that overexpressing GPAT in liver causes hepatic steatosis and hepatic insulin resistance in the absence of obesity or high-fat feeding Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
hepatic overexpression of the enzyme with an adenoviral construct Rattus norvegicus
recombinant GPAT1-FLAG adenovirus and Ad-EGFP are constructed Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + sn-glycerol 3-phosphate Rattus norvegicus
-
 CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-
liver hepatic overexpression of glycerol-sn-3-phosphate acyltransferase 1 in rats causes insulin resistance Rattus norvegicus
-
muscle
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0003
-
 activity in rat liver membranes after inhibiting endoplasmic reticulum GPAT with N-ethylmaleimide, rats are treated with Ad-EGFP Rattus norvegicus
0.00083
-
 activity in rat liver membranes after inhibiting endoplasmic reticulum GPAT with N-ethylmaleimide, rats are treated with Ad-GPAT1 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + sn-glycerol 3-phosphate
-
 Rattus norvegicus CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
fatty acyl-CoA + glycerol-3-phosphate
-
 Rattus norvegicus CoA + lysophosphatidic acid
-
 ?

Synonyms

Synonyms Comment Organism
acyl-CoA:glycerol-3-phosphate acyltransferase 1
-
 Rattus norvegicus
Ad-GPAT1 adenoviral GPAT1 construct Rattus norvegicus
glycerol-sn-3-phosphate acyltransferase 1
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 activity assay at room temperature Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 activity assay Rattus norvegicus