Literature summary for 2.3.1.142 extracted from

Mitchell, D.A.; Hamel, L.D.; Ishizuka, K.; Mitchell, G.; Schaefer, L.M.; Deschenes, R.J.
The erf4 subunit of the yeast ras palmitoyl acyltransferase is required for stability of the acyl-erf2 intermediate and palmitoyl transfer to a ras2 substrate (2012), J. Biol. Chem., 287, 34337-34348.

Search for more literature for 2.3.1.142

Cloned(Commentary)

Cloned (Comment)	Organism
co-overexpression of His-tagged Erf2 with fused FLAG-tagged Erf4 in strain RJY1827, expression of FLAG-tagged Erf2 in strain RJY	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a fusion protein that places the 58 amino acids of Erf2 onto the C-terminus of Pfa4, another DHHC enzyme that resides in the endoplasmic reticulum membrane, the modification highly reduces the half-life of the engineered protein	Saccharomyces cerevisiae

General Stability

General Stability	Organism
Erf4 and the C-terminal 58 amino acids of Erf2 are required for Erf2 stability	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum	the Erf2-Erf4 complex localizes to the endoplasmic reticulum where it palmitoylates Ras. Role of the quality control system in degrading Erf2 molecules that are not in complex with Erf4	Saccharomyces cerevisiae	5783	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
palmitoyl-CoA + mucus glycoprotein	Saccharomyces cerevisiae	protein S-palmitoylation is a posttranslational modification in which a palmitoyl group is added to a protein via a thioester linkage on cysteine	CoA + O-palmitoylglycoprotein	-	r

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	diverse strains	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged Erf2 with fused FLAG-tagged Erf4 from strain RJY1827, solubilization by 0.8% dodecylmaltoside and urea, and ultracentrifugation, purification by nickel affinity chromatography and desalting gel filtration, recombinant FLAG-tagged Erf2 by the same method	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
palmitoyl-CoA + mucus glycoprotein	protein S-palmitoylation is a posttranslational modification in which a palmitoyl group is added to a protein via a thioester linkage on cysteine	Saccharomyces cerevisiae	CoA + O-palmitoylglycoprotein	-	r
palmitoyl-CoA + mucus glycoprotein	purified recombinant His6:Erf2-Erf4 complex	Saccharomyces cerevisiae	CoA + O-palmitoylglycoprotein	-	r

Synonyms

Synonyms	Comment	Organism
Pat	-	Saccharomyces cerevisiae
ras palmitoyl acyltransferase	-	Saccharomyces cerevisiae
Ras PAT	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.8	-	assay at	Saccharomyces cerevisiae

General Information

General Information	Comment	Organism
malfunction	in the absence of Erf4, the rate of hydrolysis of the active site palmitoyl thioester intermediate is increased, resulting in reduced palmitoyl transfer to a Ras2 substrate	Saccharomyces cerevisiae
additional information	Erf4 and the C-terminal 58 amino acids of Erf2 are required for Erf2 stability. Role of the quality control system in degrading Erf2 molecules that are not in complex with Erf4. The C-terminal 58-amino acid residues of Erf2 are necessary and sufficient for ubiquitin-dependent, ERAD-mediated clearance of DHHC PATs	Saccharomyces cerevisiae
physiological function	palmitoylation is a reversible modification involved in protein membrane targeting, receptor trafficking and signaling, vesicular biogenesis and trafficking, protein aggregation, and protein degradation. An example of the dynamic nature of this modification is the palmitoylation-depalmitoylation cycle that regulates the subcellular trafficking of Ras family GTPases. The Ras protein acyltransferase consists of a complex of Erf2-Erf4 in yeast cells. Both subunits Erf2 and Erf4 are required for PAT activity, one role of Erf4 is to regulate Erf2 stability through an ubiquitin-mediated pathway. Erf4 is required for the stable formation of the palmitoyl-Erf2 intermediate, the first step of palmitoyl transfer to protein substrates	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)