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Literature summary for 2.3.1.142 extracted from
- Characterization and partial purification of protein fatty acyltransferase activity from rat liver (2003), Biochim. Biophys. Acta, 1635, 10-19.
General Stability
| General Stability | Organism |
|---|---|
| enzyme is inactivated by degrading or denaturaring agents, instable during final gel filtration purification step | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydroxylamine | - |
Rattus norvegicus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | - |
Rattus norvegicus | 5886 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | acylation of proteins through the addition of palmitate to a cysteine residue is a common posttranslational modification for a variety of proteins | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male CD strain rats | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, 95fold from liver by preparation of plasma membrane fraction, solubilization with 0.15% Triton X-100, and sequential chromatography including affinity chromatography on a cerulin-based palmitoylation inhibitor ligand | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
| additional information | the addition of different long-chain fatty acids to proteins is cell-type specific | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.4 | - |
liver plasma membrane preparation | Rattus norvegicus |
| 228 | - |
partially purified enzyme | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, partially purified enzyme, 0.15% v/v Triton X-100, 150 mM KCl, 20 mM Tris-HCl, pH 7.4, 1 mM EGTA, 0.01 mg/ml leupeptin, at least 3 months without loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | acylation of proteins through the addition of palmitate to a cysteine residue is a common posttranslational modification for a variety of proteins | Rattus norvegicus | ? | - |
? | |
| additional information | the enzyme shows a preference for palmitate and to a lesser degree for other long-chain fatty acids, overview | Rattus norvegicus | ? | - |
? | |
| RGS16 + palmitoyl-CoA | RGS16 is the regulator of G protein signaling 16 | Rattus norvegicus | O-palmitoyl-RGS16 + CoA | - |
r | |
| tubulin Galphai + palmitoyl-CoA | - |
Rattus norvegicus | O-palmitoyl-tubulin Galphai + CoA | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pat | - |
Rattus norvegicus |
| PATI | - |
Rattus norvegicus |
| protein fatty acyltransferase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
5 min, partial inactivation | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Rattus norvegicus |
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Release 2023.1 (January 2023)
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