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Literature summary for 2.3.1.142 extracted from

  • Basar, T.; Havlicek, V.; Bezouskova, S.; Hackett, M.; Sebo, P.
    Acylation of lysine 983 is sufficient for toxin activity of Bordetella pertussis adenylate cyclase. Substitutions of alanine 140 modulate acylation site selectivity of the toxin acyltransferase CyaC (2001), J. Biol. Chem., 276, 348-354.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type CyaC acyltransferase and various mutants in Escherichia coli Bordetella pertussis

Protein Variants

Protein Variants Comment Organism
A140G changed selectivity of acyltransferase for the 2 acetylation sites of adenylate cyclase toxin, a mixture of bi and monoacetylated toxins modified either at both Lys860 and Lys983 or only at Lys860 is generated Bordetella pertussis
A140V monoacetylation of adenylate cyclase toxin almost exclusively at Lys983 Bordetella pertussis
C67S no effect on adenylate cyclase toxin activation Bordetella pertussis
H33D loss of adenlayte cyclase toxin acetylation activity Bordetella pertussis
H33S loss of adenlayte cyclase toxin acetylation activity Bordetella pertussis
R141K no effect on adenylate cyclase toxin activation Bordetella pertussis
R141L no effect on adenylate cyclase toxin activation Bordetella pertussis
S30R loss of adenlayte cyclase toxin acetylation activity Bordetella pertussis
S30W loss of adenlayte cyclase toxin acetylation activity Bordetella pertussis
S68T no effect on adenylate cyclase toxin activation Bordetella pertussis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
palmitoyl-CoA + adenylate cyclase toxin Bordetella pertussis palmitoylation activates adenylate cyclase toxin CoA + palmitoyladenylate cyclase toxin
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Organism

Organism UniProt Comment Textmining
Bordetella pertussis
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-CoA + adenylate cyclase toxin acyltransferase CyaC palmitoylates the conserved lysines 983 and 860 of adenylate cyclase toxin Bordetella pertussis CoA + palmitoyladenylate cyclase toxin
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palmitoyl-CoA + adenylate cyclase toxin palmitoylation activates adenylate cyclase toxin Bordetella pertussis CoA + palmitoyladenylate cyclase toxin
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?