Protein Variants | Comment | Organism |
---|---|---|
R518N | site-directed mutagenesis, mutant shows 1650fold increased Km for L-carnitine, only slightly affected Km for acyl-CoA and kcat compared to the wild-type enzyme | Bos taurus |
S552A | site-directed mutagenesis, mutant shows 17fold increased Km for L-carnitine compared to the wild-type enzyme | Bos taurus |
S554X | site-directed mutagenesis, mutant shows 10fold decreased kcat compared to the wild-type enzyme | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Bos taurus | 16020 | - |
mitochondrion | - |
Bos taurus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
octanoyl-CoA + L-carnitine | Bos taurus | step in the transport of fatty acids across the inner mitochondrial membrane, reaction plays a role in balancing cellular CoA:acyl-CoA ratio | CoA + L-octanoylcarnitine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
octanoyl-CoA + L-carnitine = CoA + L-octanoylcarnitine | catalytic mechanism, active site structure, substrate recognition mechanism, amino acid residues 343, 564 and 553 determine the acyl-CoA substrate specificity, catalytic residue is His343 | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
octanoyl-CoA + L-carnitine | step in the transport of fatty acids across the inner mitochondrial membrane, reaction plays a role in balancing cellular CoA:acyl-CoA ratio | Bos taurus | CoA + L-octanoylcarnitine | - |
r | |
octanoyl-CoA + L-carnitine | carnitine binding site structure involving the highly conserved R518, Y452 and T465, CoA binding site n the active site tunnel via His343, fatty acid binding site | Bos taurus | CoA + L-octanoylcarnitine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
carnitine octanoyltransferase | - |
Bos taurus |
CrOT | - |
Bos taurus |
More | enzyme belongs to the family of carnitine acyltransferases | Bos taurus |