Cloned (Comment) | Organism |
---|---|
stable expression of GFP-tagged LRAT in CHO cells and of GFP-tagged LRAT in COS7 cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | before initiation of retinyl ester biosynthesis, LRAT distributes throughout the endoplasmic reticulum, and Crpb1 localizes with mitochondria associated membranes, surrounded by LRAT. Upon initiating retinyl ester biosynthesis in cells, Crpb1 remains with MAM, and both Crbp1 and MAM re-localize with LRAT. LRAT formed rings around the growing lipid droplets | Homo sapiens | 5783 | - |
lipid droplet | upon initiating retinyl ester biosynthesis in cells, Crpb1 remains with MAM, and both Crbp1 and MAM re-localize with LRAT. LRAT formed rings around the growing lipid droplets. LRAT-containing rings colocalize with the lipid-droplet surface proteins, desnutrin/adipose triglyceride lipase and perilipin 2. Colocalization with lipid droplets requires the 38 N-terminal amino acid residues of LRAT, and specifically K36 and R38. Formation of rings around the growing lipid droplets does not require functional microtubules | Homo sapiens | 5811 | - |
additional information | subcellular localization study, overview | Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + retinol-[cellular-retinol-binding-protein] | Homo sapiens | - |
2-acylglycerophosphocholine + retinyl-ester-[cellular-retinol-binding-protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + retinol-[cellular-retinol-binding-protein] | - |
Homo sapiens | 2-acylglycerophosphocholine + retinyl-ester-[cellular-retinol-binding-protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lecithin:retinol acyltransferase | - |
Homo sapiens |
LRAT | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | relationship between LRAT and Crbp1 during retinyl ester biosynthesis in which mitochondria associated membranes-associated Crpb1 and LRAT colocalize, and both surround the growing retinyl ester-containing lipid droplet. The N-terminus of LRAT, especially K36 and R38, is essential to colocalization with the lipid droplet | Homo sapiens |