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Literature summary for 2.3.1.135 extracted from

  • Kaschula, C.H.; Jin, M.H.; Desmond-Smith, N.S.; Travis, G.H.
    Acyl CoA:retinol acyltransferase (ARAT) activity is present in bovine retinal pigment epithelium (2005), Exp. Eye Res., 82, 111-121.
    View publication on PubMed
Search for more literature for 2.3.1.135

Cloned(Commentary)

Cloned (Comment) Organism
stable expression of LRAT in HEK-293T cell membranes Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
all-trans-retinylbromoacetate
-
 Bos taurus
additional information no inhibition by progesterone Bos taurus
palmitoyl-CoA
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Bos taurus
0.00033
-
 all-trans-retinol-[cellular retinol-binding protein] pH 7.5, 37°C, retinal pigment epithelium microsomes Bos taurus
0.0023
-
 11-cis-retinol-[cellular retinol-binding protein] pH 7.5, 37°C, retinal pigment epithelium microsomes Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Bos taurus 16020
-
microsome
-
 Bos taurus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 x * 25000, recombinant enzyme Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lecithin + 11-cis-retinol-[cellular retinol-binding protein] Bos taurus
-
 2-acylglycerophosphocholine + 11-cis-retinyl ester-[cellular retinol-binding protein]
-
 r
lecithin + all-trans-retinol-[cellular retinol-binding protein] Bos taurus
-
 2-acylglycerophosphocholine + all-trans-retinyl ester-[cellular retinol-binding protein]
-
 r
additional information Bos taurus ARAT, EC 2.3.1.76, may complement LRAT to provide additional retinyl-ester synthase activity under conditions of high all-trans-retinol ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially by microsome preparation from eyes, recombinant enzyme partially from HEK-293T cells by membrane preparation Bos taurus

Reaction

Reaction Comment Organism Reaction ID
phosphatidylcholine + retinol-[cellular-retinol-binding-protein] = 2-acylglycerophosphocholine + retinyl-ester-[cellular-retinol-binding-protein] enzyme mechanism and role in the visual cycle Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
eye
-
 Bos taurus
-
retinal pigment epithelium
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lecithin + 11-cis-retinol-[cellular retinol-binding protein]
-
 Bos taurus 2-acylglycerophosphocholine + 11-cis-retinyl ester-[cellular retinol-binding protein]
-
 r
lecithin + all-trans-retinol-[cellular retinol-binding protein]
-
 Bos taurus 2-acylglycerophosphocholine + all-trans-retinyl ester-[cellular retinol-binding protein]
-
 r
additional information ARAT, EC 2.3.1.76, may complement LRAT to provide additional retinyl-ester synthase activity under conditions of high all-trans-retinol Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 25000, recombinant enzyme Bos taurus

Synonyms

Synonyms Comment Organism
lecithin:retinol acyltransferase
-
 Bos taurus
LRAT
-
 Bos taurus
More cf. EC 2.3.1.76 Bos taurus
retinyl-ester synthase
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 assay at Bos taurus