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Literature summary for 2.3.1.133 extracted from
- Hydroxycinnamoyl-CoA: transferases in higher plants. II. Characterization in Cichorium endivia and Raphanus sativus and comparison with other plants (1994), Plant Physiol. Biochem., 32, 355-363.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | no effect | Raphanus sativus |  |
| EDTA | no effect | Cichorium endivia | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibition at 5 mM | Cichorium endivia | |
| Ca2+ | inhibition at 5 mM | Raphanus sativus | |
| DTT | concentration above 5 mM results in a marked inhibition of activity | Cichorium endivia | |
| DTT | concentration above 5 mM results in a marked inhibition of activity | Raphanus sativus | |
| Mg2+ | inhibition at 5 mM | Cichorium endivia | |
| Mg2+ | inhibition at 5 mM | Raphanus sativus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12.5 | - |
caffeoyl-CoA | - |
Cichorium endivia | |
| 14.3 | - |
4-coumaroyl-CoA | - |
Raphanus sativus | |
| 14.3 | - |
4-coumaroyl-CoA | - |
Cichorium endivia | |
| 15.4 | - |
feruloyl-CoA | - |
Cichorium endivia | |
| 30.5 | - |
caffeoyl-CoA | - |
Raphanus sativus | |
| 55.5 | - |
feruloyl-CoA | - |
Raphanus sativus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | sligthly stimulated by 3.0 mM, inhibition at 5 mM | Raphanus sativus | |
| Ca2+ | sligthly stimulated by 3.0 mM, inhibition at 5 mM | Cichorium endivia | |
| Mg2+ | slightly stimulated by 0.5 mM, inhibition at 5 mM | Raphanus sativus | |
| Mg2+ | slightly stimulated by 0.5 mM, inhibition at 5 mM | Cichorium endivia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
gel filtration | Raphanus sativus |
| 40000 | - |
gel filtration | Cichorium endivia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-coumaroyl-CoA + shikimate | Raphanus sativus | hydroxycinnamic acid-shikimate ester biosynthesis | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
| 4-coumaroyl-CoA + shikimate | Cichorium endivia | hydroxycinnamic acid-shikimate ester biosynthesis | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cichorium endivia | - |
- |
- |
| Raphanus sativus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cichorium endivia |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Raphanus sativus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Cichorium endivia |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, for several months, no apparent loss of activity | Cichorium endivia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-coumaroyl-CoA + quinate | - |
Raphanus sativus | CoA + 4-coumaroylquinate | - |
? | |
| 4-coumaroyl-CoA + shikimate | - |
Raphanus sativus | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
| 4-coumaroyl-CoA + shikimate | - |
Cichorium endivia | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
| 4-coumaroyl-CoA + shikimate | hydroxycinnamic acid-shikimate ester biosynthesis | Raphanus sativus | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
| 4-coumaroyl-CoA + shikimate | hydroxycinnamic acid-shikimate ester biosynthesis | Cichorium endivia | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
| caffeoyl-CoA + shikimate | - |
Raphanus sativus | CoA + 5-O-caffeoylshikimate | - |
? | |
| caffeoyl-CoA + shikimate | - |
Cichorium endivia | CoA + 5-O-caffeoylshikimate | - |
? | |
| feruloyl-CoA + shikimate | - |
Raphanus sativus | CoA + 5-O-feruloylshikimate | - |
? | |
| feruloyl-CoA + shikimate | - |
Cichorium endivia | CoA + 5-O-feruloylshikimate | - |
? | |
| additional information | no substrate: cinnamoyl-CoA, L-malate, D(+)-malate, L(+)-tartrate, m-tartrate, citrate, glucose, UDP-glucose, myo-inositol, tyramine, agmatine | Raphanus sativus | ? | - |
? | |
| additional information | no substrate: cinnamoyl-CoA, L-malate, D(+)-malate, L(+)-tartrate, m-tartrate, citrate, glucose, UDP-glucose, myo-inositol, tyramine, agmatine | Cichorium endivia | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
50 mM potassium phosphate buffer | Cichorium endivia |
| 6.5 | - |
pI: 4.33 | Cichorium endivia |
| 6.8 | - |
50 mM potassium phosphate buffer | Raphanus sativus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Raphanus sativus |
| 5 | 9 | - |
Cichorium endivia |
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