Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | activation | Secale cereale |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
4-coumaroyl-CoA | - |
Secale cereale | |
0.045 | - |
caffeoyl-CoA | - |
Secale cereale | |
0.59 | - |
shikimate | with 4-coumaroyl-CoA | Secale cereale | |
83 | - |
shikimate | with caffeoyl-CoA | Secale cereale |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Secale cereale | - |
rye, var. Kustro | - |
Purification (Comment) | Organism |
---|---|
partial | Secale cereale |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | primary | Secale cereale | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0056 | - |
- |
Secale cereale |
Storage Stability | Organism |
---|---|
-20°C, at least 6 months | Secale cereale |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + shikimate | best substrate | Secale cereale | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
caffeoyl-CoA + shikimate | condensation at 52% the rate of the reaction with p-coumaroyl-CoA | Secale cereale | CoA + 5-O-caffeoylshikimate | - |
? | |
feruloyl-CoA + shikimate | condensation at 22% the rate of the reaction with p-coumaroyl-CoA | Secale cereale | CoA + 5-O-feruloylshikimate | - |
? | |
additional information | 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate | Secale cereale | ? | - |
? | |
sinapoyl-CoA + shikimate | condensation at 29% the rate of the reaction with p-coumaroyl-CoA | Secale cereale | CoA + 5-O-sinapoylshikimate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
denaturation after 5 min | Secale cereale |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
200 mM potassium phosphate buffer | Secale cereale |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.3 | 7.7 | about 80% of maximal activity at pH 6.3 and 7.7 | Secale cereale |