Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | activates | Secale cereale |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
sinapoyl-CoA | - |
Secale cereale | |
10 | - |
glucarolactone | - |
Secale cereale |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sinapoyl-CoA + glucarolactone | Secale cereale | best substrate | CoA + O-sinapoylglucarolactone | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Secale cereale | - |
rye, var. Kustro | - |
Purification (Comment) | Organism |
---|---|
partial | Secale cereale |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
primary leaf | - |
Secale cereale | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.022 | - |
- |
Secale cereale |
Storage Stability | Organism |
---|---|
-20°C, at least 6 months | Secale cereale |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
caffeoyl-CoA + glucarolactone | poor substrate | Secale cereale | CoA + O-caffeoylglucarolactone | - |
? | |
feruloyl-CoA + glucarolactone | condensation at 37% the rate of the reaction with sinapoyl-CoA | Secale cereale | CoA + O-feruloylglucarolactone | - |
? | |
additional information | no activity with 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate | Secale cereale | ? | - |
? | |
p-coumaroyl-CoA + glucarolactone | condensation at 54% the rate of the reaction with sinapoyl-CoA | Secale cereale | CoA + O-p-coumaroylglucarolactone | - |
? | |
sinapoyl-CoA + glucarolactone | best substrate | Secale cereale | CoA + O-sinapoylglucarolactone | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
denaturation after 5 min | Secale cereale |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
50 mM potassium phosphate buffer | Secale cereale |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.4 | 8.2 | about 80% of maximal activity at pH 7.4 and pH 8.2 | Secale cereale |