Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.129 extracted from

  • Gong, H.; Murphy, A.; McMaster, C.R.; Byers, D.M.
    Neutralization of acidic residues in helix II stabilizes the folded conformation of acyl carrier protein and variably alters its function with different enzymes (2007), J. Biol. Chem., 282, 4494-4503.
    View publication on PubMed
Search for more literature for 2.3.1.129

Inhibitors

Inhibitors Comment Organism Structure
myristoyl-[acyl-carrier protein]
-
 Vibrio harveyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the kinetic analysis of LpxA is complicated by the thermodynamically unfavorable reaction in the forward direction, overview Vibrio harveyi

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ not as effective as Mg2+ Vibrio harveyi
Mg2+ is required for stabilization of substrate acyl-carrier protein in a helical conformation Vibrio harveyi
Mn2+ not as effective as Mg2+ Vibrio harveyi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine Vibrio harveyi
-
 [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Vibrio harveyi
-
 gene lpxA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine
-
 Vibrio harveyi [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
 ?
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine i.e. (R)-3-hydroxymyristoyl-[acyl-carrier protein], the forward reaction direction is thermodynamically unfavorable, recombinant His-tagged enzyme, the activity with mutant acyl-carrier proteins is impaired, especially with mutations of the B-site of the acyl-carrier protein, recombinant wild-type and mutant ACP substrates, overview Vibrio harveyi [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
 ?

Synonyms

Synonyms Comment Organism
LpxA
-
 Vibrio harveyi
UDP-N-acetylglucosamine acyltransferase
-
 Vibrio harveyi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Vibrio harveyi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Vibrio harveyi