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Literature summary for 2.3.1.125 extracted from
- Synthesis of a novel acetylated neutral lipid related to platelet-activating factor by acyl-CoA:1-O-alkyl-2-acetyl-sn-glycerol acyltransferase in HL-60 cells [published erratum appears in J Biol Chem 1988 Jul 25;263(21):10539] (1988), J. Biol. Chem., 263, 2593-2596.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | activation, molar ratio substrate linoleoyl-CoA/albumin: 2:4:1 | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-O-Hexadecyl-2-oleoyl-sn-glycerol | competitive inhibitor of 1-hexadecyl-2-acetyl-3-acyl-sn-glycerol synthesis | Homo sapiens |  |
| 1-oleoyl-2-acetyl-sn-glycerol | mixed-type inhibition | Homo sapiens | |
| Ca2+ | 6% inhibition at 2 mM | Homo sapiens | |
| Cu2+ | 96% inhibition at 2 mM | Homo sapiens | |
| dithiothreitol | - |
Homo sapiens | |
| Mn2+ | 35% inhibition at 2 mM | Homo sapiens | |
| Zn2+ | 93% inhibition at 2 mM | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0085 | - |
linoleoyl-CoA | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no metal ion requirement | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | related to platelet-activating factor metabolism | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HL-60 cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| linoleoyl-CoA + 1-O-hexadecyl-2-acetyl-sn-glycerol | best substrate, not acetyl-CoA | Homo sapiens | CoA + 1-O-hexadecyl-2-acetyl-3-linoleoyl-sn-glycerol | - |
? | |
| additional information | related to platelet-activating factor metabolism | Homo sapiens | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
stable below | Homo sapiens |
| 58 | - |
50% activity after 15 min | Homo sapiens |
| 65 | - |
inactivation | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.032 | - |
1-O-Hexadecyl-2-oleoyl-sn-glycerol | - |
Homo sapiens | |
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