Literature summary for 2.3.1.12 extracted from

Chandrasekhar, K.; Wang, J.; Arjunan, P.; Sax, M.; Park, Y.H.; Nemeria, N.S.; Kumaran, S.; Song, J.; Jordan, F.; Furey, W.
Insight to the interaction of the dihydrolipoamide acetyltransferase (E2) core with the peripheral components in the Escherichia coli pyruvate dehydrogenase complex via multifaceted structural approaches (2013), J. Biol. Chem., 288, 15402-15417.

Search for more literature for 2.3.1.12

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
K191E	the mutant shows 50% activity compared to the wild type enzyme	Escherichia coli
K191R	the mutant shows 79% activity compared to the wild type enzyme	Escherichia coli
R129A	the mutant shows 34% activity compared to the wild type enzyme	Escherichia coli
R129E	the mutant shows 3.0% activity compared to the wild type enzyme	Escherichia coli
R129K	the mutant shows 77% activity compared to the wild type enzyme	Escherichia coli
R130A	the mutant shows wild type activity	Escherichia coli
R130E	the mutant shows 57% activity compared to the wild type enzyme	Escherichia coli
R150A	the mutant shows 8.0% activity compared to the wild type enzyme	Escherichia coli
R150E	the mutant shows 2.4% activity compared to the wild type enzyme	Escherichia coli
R150K	the mutant shows 5.0% activity compared to the wild type enzyme	Escherichia coli
R153E	the mutant shows 60% activity compared to the wild type enzyme	Escherichia coli
R153K	the mutant shows 83% activity compared to the wild type enzyme	Escherichia coli
R161A	the mutant shows 65% activity compared to the wild type enzyme	Escherichia coli
R161E	the mutant shows 84% activity compared to the wild type enzyme	Escherichia coli
R202E	the mutant shows 10% activity compared to the wild type enzyme	Escherichia coli
R202K	the mutant shows 86% activity compared to the wild type enzyme	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
65959	-	x * 65959, calculated from amino acid sequence	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine	Escherichia coli	-	CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P06959	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel affinity column chromatography	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine	-	Escherichia coli	CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine	-	?

Subunits

Subunits	Comment	Organism
?	x * 65959, calculated from amino acid sequence	Escherichia coli

Synonyms

Synonyms	Comment	Organism
dihydrolipoamide acetyltransferase	-	Escherichia coli
pyruvate dehydrogenase complex component E2	-	Escherichia coli

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Release 2023.1 (January 2023)